Infectious Agents Identified by Real-Time PCR, Serology and Bacteriology in Blood and Peritoneal Exudate Samples of Cows Affected by Parietal Fibrinous Peritonitis after Caesarean Section
The aim of this study was to identify the pathogens potentially involved in parietal fibrinous peritonitis (PFP). PFP is a complication of laparotomy in cattle, characterized by an accumulation of exudate inside a fibrinous capsule. We have studied 72 cases of PFP in Belgian blue cows, confirmed by a standard diagnostic protocol. Blood was collected to evaluate the presence of antibodies for Mycoplasma bovis(M. bovis), Coxiella burnetii(C. burnetii) and Bovine Herpesvirus 4(BoHV4) by enzyme-linked immunosorbent assays.
Peritoneal exudate was obtained from the PFP cavity to perform bacteriological culture, and to identify the DNA of M. bovis, C. burnetii and BoHV4 using real time polymerase chain reaction (qPCR). Bacteriological culture was positive in most peritoneal samples (59/72); Trueperella pyogenes (T. pyogenes) (51/72) and Escherichia coli (E. coli) (20/72) were the most frequently identified. For BoHV4, the majority of cows showed positive serology and qPCR (56/72 and 49/72, respectively).
Contrariwise, M. bovis (17/72 and 6/72, respectively) and C. burnetii (15/72 and 6/72, respectively) were less frequently detected (p < 0.0001). Our study proves that PFP can no longer be qualified as a sterile inflammation. Moreover, we herein describe the first identification of BoHV4 and C. burnetii in cows affected by PFP.
Cyclopiazonic acid |
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C192-1MG | TOKU-E | 1 mg | EUR 135.6 |
Cyclopiazonic acid |
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C192-5MG | TOKU-E | 5 mg | EUR 382.8 |
Lithocholic Acid |
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A8463-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 135.6 |
Description: Lithocholic acid (LCA) is a toxic secondary bile acid, causing intrahepatic cholestasis, which has tumor-promoting activity. |
Lithocholic Acid |
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A8463-50 | ApexBio | 50 mg | EUR 157.2 |
Description: Lithocholic acid (LCA) is a toxic secondary bile acid, causing intrahepatic cholestasis, which has tumor-promoting activity. |
Piromidic Acid |
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A8502-10 | ApexBio | 10 mg | EUR 129.6 |
Description: Shown to have antibacterial properties. |
Piromidic Acid |
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A8502-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 129.6 |
Description: Shown to have antibacterial properties. |
Piromidic Acid |
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A8502-50 | ApexBio | 50 mg | EUR 199.2 |
Description: Shown to have antibacterial properties. |
Dihydroaeruginoic acid |
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D052-25MG | TOKU-E | 25 mg | EUR 804 |
Dihydroaeruginoic acid |
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D052-5MG | TOKU-E | 5 mg | EUR 255.6 |
Dipicolinic acid |
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D084-25MG | TOKU-E | 25 mg | EUR 285.6 |
Dipicolinic acid |
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D084-5MG | TOKU-E | 5 mg | EUR 106.8 |
Penicillic Acid |
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C3438-10 | ApexBio | 10 mg | EUR 170.4 |
Description: Penicillic acid is synthesized by a large number of fungi, such as P. puberulum, Penicillium stoloniferum, P. eye/opium Penicillium martensii, Penicillium thomii. The synthesis of this secondary metabolite has little or no taxonomic significance [1]. |
Penicillic Acid |
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C3438-25 | ApexBio | 25 mg | EUR 324 |
Description: Penicillic acid is synthesized by a large number of fungi, such as P. puberulum, Penicillium stoloniferum, P. eye/opium Penicillium martensii, Penicillium thomii. The synthesis of this secondary metabolite has little or no taxonomic significance [1]. |
Ascorbic Acid |
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CA052-010 | GenDepot | 100g | EUR 92.4 |
Ascorbic Acid |
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CA052-050 | GenDepot | 500g | EUR 157.2 |
Fusaric Acid |
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C5062-250 | ApexBio | 250 mg | EUR 174 |
Description: Fusaric acid is a mycotoxin produced by several species of Fusarium [1]. Mycotoxins are biologically active secondary fungal metabolites found as contaminants of food- and feedstuff. Mycotoxin is capable of causing disease and death in both humans and animals. |
Fusaric Acid |
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C5062-500 | ApexBio | 500 mg | EUR 268.8 |
Description: Fusaric acid is a mycotoxin produced by several species of Fusarium [1]. Mycotoxins are biologically active secondary fungal metabolites found as contaminants of food- and feedstuff. Mycotoxin is capable of causing disease and death in both humans and animals. |
Tienilic Acid |
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C5345-10 | ApexBio | 10 mg | EUR 223.2 |
Description: Km: 5 microM for CYP 2C9 catalyzing 5-hydroxylation of tienilic acid Tienilic acid is a specific suicide substrate for CYP2C9 and CYP2C10.Cytochrome P450 enzymes function to metabolize both endogenous and exogenous xenobiotics. |
Tienilic Acid |
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C5345-25 | ApexBio | 25 mg | EUR 495.6 |
Description: Km: 5 microM for CYP 2C9 catalyzing 5-hydroxylation of tienilic acid Tienilic acid is a specific suicide substrate for CYP2C9 and CYP2C10.Cytochrome P450 enzymes function to metabolize both endogenous and exogenous xenobiotics. |
Tienilic Acid |
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C5345-5 | ApexBio | 5 mg | EUR 157.2 |
Description: Km: 5 microM for CYP 2C9 catalyzing 5-hydroxylation of tienilic acid Tienilic acid is a specific suicide substrate for CYP2C9 and CYP2C10.Cytochrome P450 enzymes function to metabolize both endogenous and exogenous xenobiotics. |
Tienilic Acid |
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C5345-50 | ApexBio | 50 mg | EUR 834 |
Description: Km: 5 microM for CYP 2C9 catalyzing 5-hydroxylation of tienilic acid Tienilic acid is a specific suicide substrate for CYP2C9 and CYP2C10.Cytochrome P450 enzymes function to metabolize both endogenous and exogenous xenobiotics. |
Eicosatetraynoic Acid |
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C5515-10 | ApexBio | 10 mg | EUR 189.6 |
Description: Eicosatetraynoic Acid is nonspecific inhibitor of cyclooxygenases and lipoxygenases [1]. Cyclooxygenase (COX) is the key enzyme required for the conversion of arachidonic acid to prostaglandins. |
Eicosatetraynoic Acid |
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C5515-25 | ApexBio | 25 mg | EUR 369.6 |
Description: Eicosatetraynoic Acid is nonspecific inhibitor of cyclooxygenases and lipoxygenases [1]. Cyclooxygenase (COX) is the key enzyme required for the conversion of arachidonic acid to prostaglandins. |
Eicosatetraynoic Acid |
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C5515-50 | ApexBio | 50 mg | EUR 607.2 |
Description: Eicosatetraynoic Acid is nonspecific inhibitor of cyclooxygenases and lipoxygenases [1]. Cyclooxygenase (COX) is the key enzyme required for the conversion of arachidonic acid to prostaglandins. |
?-Linolenic Acid |
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C5518-1000 | ApexBio | 1 g | EUR 313.2 |
Description: Gamma-linolenic acid is a weak Leukotriene B4 (LTB4) receptor antagonist.Gamma-linolenic acid (GLA), an omega-6 fatty acid, is an essential fatty acid necessary for human health. You have to get them through food. |
?-Linolenic Acid |
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C5518-250 | ApexBio | 250 mg | EUR 164.4 |
Description: Gamma-linolenic acid is a weak Leukotriene B4 (LTB4) receptor antagonist.Gamma-linolenic acid (GLA), an omega-6 fatty acid, is an essential fatty acid necessary for human health. You have to get them through food. |
Evernic Acid |
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C5610-100 | ApexBio | 100 mg | EUR 486 |
Description: Evernic Acid is an inhibitor of two key plasmodial FAS-II enzymes PfFabZ and PfFabIThe Type II fatty acid synthase (FAS) system catalysed fatty acid biosynthesis in most bacteria. |
Evernic Acid |
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C5610-250 | ApexBio | 250 mg | EUR 912 |
Description: Evernic Acid is an inhibitor of two key plasmodial FAS-II enzymes PfFabZ and PfFabIThe Type II fatty acid synthase (FAS) system catalysed fatty acid biosynthesis in most bacteria. |
Evernic Acid |
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C5610-50 | ApexBio | 50 mg | EUR 315.6 |
Description: Evernic Acid is an inhibitor of two key plasmodial FAS-II enzymes PfFabZ and PfFabIThe Type II fatty acid synthase (FAS) system catalysed fatty acid biosynthesis in most bacteria. |
?-Guanidinoglutaric Acid |
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C5708-100 | ApexBio | 100 mg | EUR 549.6 |
Description: ?-Guanidinoglutaric Acid is a novel nitric oxide synthase inhibitor with Ki value of 2.69 ?M [1]. |
?-Guanidinoglutaric Acid |
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C5708-50 | ApexBio | 50 mg | EUR 339.6 |
Description: ?-Guanidinoglutaric Acid is a novel nitric oxide synthase inhibitor with Ki value of 2.69 ?M [1]. |
Folic Acid |
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AG062 | Unibiotest | 1 mg | EUR 627.6 |
Ferulic Acid |
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AG130 | Unibiotest | 1 mg | EUR 627.6 |
Benzoic Acid |
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AG153 | Unibiotest | 1 mg | EUR 627.6 |
Trichlorophenoxyacetic Acid |
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AG169 | Unibiotest | 1 mg | EUR 627.6 |
Tenuazonic acid |
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AG183 | Unibiotest | 1 mg | EUR 627.6 |
Glycochenodeoxycholic acid |
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AG216 | Unibiotest | 1 mg | EUR 627.6 |
Arsanilic acid |
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AG255 | Unibiotest | 1 mg | EUR 627.6 |
Gibberellic acid |
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AG268 | Unibiotest | 1 mg | EUR 627.6 |
Asterric Acid |
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C4549-1 | ApexBio | 1 mg | EUR 351.6 |
Description: IC50: 10 ?M for ET-1 binding to the ETA receptor ofA10 cellsAsterric Acid is an ETA receptor inhibitor. Endothelin (ET), a potent vasoconstrictor/pressor peptide consisting of 21 amino acid residues, was first isolated from the cultures of porcine aortic endothelial cells. |
Asterric Acid |
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C4549-5 | ApexBio | 5 mg | EUR 1155.6 |
Description: IC50: 10 ?M for ET-1 binding to the ETA receptor ofA10 cellsAsterric Acid is an ETA receptor inhibitor. Endothelin (ET), a potent vasoconstrictor/pressor peptide consisting of 21 amino acid residues, was first isolated from the cultures of porcine aortic endothelial cells. |
Maslinic Acid |
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C4832-10 | ApexBio | 10 mg | EUR 339.6 |
Description: Maslinic acid is a pentacyclic triterpene existed in the protective wax-like coating of the leaves and fruit of Olea europaea L [1]. Maslinic acid is an inhibitor of NO, H2O2, IL-6 and TNF-?. |
Maslinic Acid |
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C4832-25 | ApexBio | 25 mg | EUR 673.2 |
Description: Maslinic acid is a pentacyclic triterpene existed in the protective wax-like coating of the leaves and fruit of Olea europaea L [1]. Maslinic acid is an inhibitor of NO, H2O2, IL-6 and TNF-?. |
Maslinic Acid |
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C4832-5 | ApexBio | 5 mg | EUR 217.2 |
Description: Maslinic acid is a pentacyclic triterpene existed in the protective wax-like coating of the leaves and fruit of Olea europaea L [1]. Maslinic acid is an inhibitor of NO, H2O2, IL-6 and TNF-?. |
Ethacrynic Acid |
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C4125-1000 | ApexBio | 1 g | EUR 145.2 |
Ethacrynic Acid |
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C4125-10000 | ApexBio | 10 g | EUR 818.4 |
Ethacrynic Acid |
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C4125-5000 | ApexBio | 5 g | EUR 459.6 |
Fusidic acid |
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F019-100MG | TOKU-E | 100 mg | EUR 100.8 |
Fusidic acid |
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F019-1G | TOKU-E | 1 g | EUR 349.2 |
Fuchsin acid |
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FB0469 | Bio Basic | 100g | EUR 118.46 |
Pressinoic Acid |
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5-01806 | CHI Scientific | 4 x 5mg | Ask for price |
Asterric Acid |
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A132-1MG | TOKU-E | 1 mg | EUR 313.2 |
Asterric Acid |
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A132-5MG | TOKU-E | 5 mg | EUR 1006.8 |
Zoledronic Acid |
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A1352-100 | ApexBio | 100 mg | EUR 268.8 |
Description: Zoledronic acid, one of the most potent nitrogen-containing bisphosphonates, induces anti-proliferative and apoptotic effects on multiple myeloma cell lines in vitro by activating protein kinase C. Zoledronic acid also inhibits proliferation of human foet |
Zoledronic Acid |
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A1352-25 | ApexBio | 25 mg | EUR 129.6 |
Description: Zoledronic acid, one of the most potent nitrogen-containing bisphosphonates, induces anti-proliferative and apoptotic effects on multiple myeloma cell lines in vitro by activating protein kinase C. Zoledronic acid also inhibits proliferation of human foet |
Zoledronic Acid |
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A1352-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 129.6 |
Description: Zoledronic acid, one of the most potent nitrogen-containing bisphosphonates, induces anti-proliferative and apoptotic effects on multiple myeloma cell lines in vitro by activating protein kinase C. Zoledronic acid also inhibits proliferation of human foet |
Anacardic Acid |
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A160-10MG | TOKU-E | 10mg | EUR 144 |
Anacardic Acid |
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A160-250MG | TOKU-E | 250mg | EUR 298.8 |
ICG acid |
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189 | AAT Bioquest | 5 mg | EUR 262.8 |
Mycophenolic acid |
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10-030 | ProSci | 500 mg | EUR 539.16 |
Description: Antibiotic [1]. Shows antiviral, antifungal and antitumor properties [1, 2]. Immunosuppressive drug used to prevent rejection in organ transplantation, rheumatoid arthritis, and psoriasis [3-6, 13]. Potent reversible inhibitor of inosine-5'-monophosphate dehydrogenase (IMPDH), leading to depletion of GMP and interruption of the de novo synthesis of purine nucleotides necessary for B and T lymphocyte proliferation [7, 8]. Inhibits the type II IMPDH isoform (IMPDH-2) 5-fold more potently compared to type I isoform [11]. Inhibits RNA and DNA synthesis [13]. Inducible nitric oxide synthase (iNOS/NOS II) inhibitor [8, 10]. Apoptosis and necrosis inducer [9, 12]. Novel type of inhibitor against RNA guanylyltransferases [14]. Inhibits TNF-α-stimulated MAPK/NF-κB and ROS generation [15]. Autophagy suppressor. Broad-spectrum antiviral compound with inhibitory activity against different coronavirus, including SARS-CoV, MERS-CoV and SARS-CoV-2, cause of COVID-19. It binds to the active site of SARS-CoV-2 papain-like protease (PLpro) possibly inhibiting viral replication. |
Betulinic acid |
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A3237-100 | ApexBio | 100 mg | EUR 129.6 |
Description: Betulinic acid is a natural pentacyclic triterpenoid that displays anti-HIV and antitumor activity. Betulinic acid selectively induces apoptosis in tumor cells by directly activating the mitochondrial pathway of apoptosis through a p53- and CD95-independent mechanism. |
Betulinic acid |
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A3237-200 | ApexBio | 200 mg | EUR 177.6 |
Description: Betulinic acid is a natural pentacyclic triterpenoid that displays anti-HIV and antitumor activity. Betulinic acid selectively induces apoptosis in tumor cells by directly activating the mitochondrial pathway of apoptosis through a p53- and CD95-independent mechanism. |
Betulinic acid |
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A3237-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 129.6 |
Description: Betulinic acid is a natural pentacyclic triterpenoid that displays anti-HIV and antitumor activity. Betulinic acid selectively induces apoptosis in tumor cells by directly activating the mitochondrial pathway of apoptosis through a p53- and CD95-independent mechanism. |
Betulinic acid |
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A3237-500 | ApexBio | 500 mg | EUR 309.6 |
Description: Betulinic acid is a natural pentacyclic triterpenoid that displays anti-HIV and antitumor activity. Betulinic acid selectively induces apoptosis in tumor cells by directly activating the mitochondrial pathway of apoptosis through a p53- and CD95-independent mechanism. |
Gambogic Acid |
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A3424-10 | ApexBio | 10 mg | EUR 192 |
Description: Gambogic acid (GA) is an inducer of apoptosis with EC50 value of 0.78-1.64 ?M for caspases and with IC50 values of 1.47, 1.21, 2.02, 0.66, 1.06 and 0.79 ?M for Bcl-XL, Bcl-2, Bcl-W, Bcl-B, Bfl-1 and Mcl-1, respectively. |
Gambogic Acid |
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A3424-25 | ApexBio | 25 mg | EUR 261.6 |
Description: Gambogic acid (GA) is an inducer of apoptosis with EC50 value of 0.78-1.64 ?M for caspases and with IC50 values of 1.47, 1.21, 2.02, 0.66, 1.06 and 0.79 ?M for Bcl-XL, Bcl-2, Bcl-W, Bcl-B, Bfl-1 and Mcl-1, respectively. |
Gambogic Acid |
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A3424-5 | ApexBio | 5 mg | EUR 150 |
Description: Gambogic acid (GA) is an inducer of apoptosis with EC50 value of 0.78-1.64 ?M for caspases and with IC50 values of 1.47, 1.21, 2.02, 0.66, 1.06 and 0.79 ?M for Bcl-XL, Bcl-2, Bcl-W, Bcl-B, Bfl-1 and Mcl-1, respectively. |
Gambogic Acid |
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A3424-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 184.8 |
Description: Gambogic acid (GA) is an inducer of apoptosis with EC50 value of 0.78-1.64 ?M for caspases and with IC50 values of 1.47, 1.21, 2.02, 0.66, 1.06 and 0.79 ?M for Bcl-XL, Bcl-2, Bcl-W, Bcl-B, Bfl-1 and Mcl-1, respectively. |
Kainic acid |
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20-abx076647 | Abbexa |
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Quisqualic Acid |
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20-abx076651 | Abbexa |
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Retinoic acid |
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20-abx076656 | Abbexa |
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Okadaic acid |
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20-abx076703 | Abbexa |
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(+)-Abscisic Acid |
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abx076833-1mg | Abbexa | 1 mg | EUR 493.2 |
Tropodithietic acid |
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abx076844-1mg | Abbexa | 1 mg | EUR 477.6 |
Abscisic Acid |
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abx082046-100mg | Abbexa | 100 mg | EUR 243.6 |
Folic acid |
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abx082263-100g | Abbexa | 100 g | EUR 376.8 |
Folic acid |
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20-abx082265 | Abbexa |
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Nicotinic acid |
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20-abx082297 | Abbexa |
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Maleic acid |
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abx082326-100g | Abbexa | 100 g | EUR 226.8 |
Pyruvic acid |
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20-abx082337 | Abbexa |
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Boric acid |
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abx082355-500g | Abbexa | 500 g | EUR 243.6 |
Gibberellic acid |
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20-abx082365 | Abbexa |
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Boric acid |
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abx082375-500g | Abbexa | 500 g | EUR 226.8 |
Pyruvic acid |
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20-abx082493 | Abbexa |
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Lipoteichoic Acid |
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abx087002-1mg | Abbexa | 1 mg | EUR 343.2 |
Calconcarboxylic Acid |
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abx090682-25g | Abbexa | 25 g | EUR 243.6 |
Carnosic acid |
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20-abx076520 | Abbexa |
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Betulinic Acid |
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20-abx076536 | Abbexa |
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Anacardic acid |
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A4488-10 | ApexBio | 10 mg | EUR 142.8 |
Description: IC50: Noncompetitively inhibit histone acetyltransferase (HAT) activity in prostate cancer with an IC50 value of about 5.0 M.Anacardic acid (AA) is commonly regarded as a non-specific HAT inhibitor of p300. |
Anacardic acid |
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A4488-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 129.6 |
Description: IC50: Noncompetitively inhibit histone acetyltransferase (HAT) activity in prostate cancer with an IC50 value of about 5.0 M.Anacardic acid (AA) is commonly regarded as a non-specific HAT inhibitor of p300. |
Anacardic acid |
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A4488-50 | ApexBio | 50 mg | EUR 393.6 |
Description: IC50: Noncompetitively inhibit histone acetyltransferase (HAT) activity in prostate cancer with an IC50 value of about 5.0 M.Anacardic acid (AA) is commonly regarded as a non-specific HAT inhibitor of p300. |
Okadaic acid |
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A4540-.05 | ApexBio | 50 µg | EUR 182.4 |
Description: Okadaic acid is a potent inhibitor of protein phosphatase 1/2A with IC50 values of 19 nM and 0.2 nM for protein phosphatase 1 and protein phosphatase 2A, respectively [1]. |
Okadaic acid |
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A4540-.1 | ApexBio | 100 µg | EUR 292.8 |
Description: Okadaic acid is a potent inhibitor of protein phosphatase 1/2A with IC50 values of 19 nM and 0.2 nM for protein phosphatase 1 and protein phosphatase 2A, respectively [1]. |
Ellagic acid |
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A2306-25 | ApexBio | 25 mg | EUR 199.2 |
Description: Selective, ATP-competitive inhibitor of casein kinase 2 (CK2) (IC50 values are 40, 2900, 3500, 4300 and 9400 nM for CK2, Lyn, PKA, Syk?and FGR respectively). Exhibits antioxidant, antitumor and anticarcinogenic activity and also inhibits glutat |
Ellagic acid |
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A2306-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 129.6 |
Description: Selective, ATP-competitive inhibitor of casein kinase 2 (CK2) (IC50 values are 40, 2900, 3500, 4300 and 9400 nM for CK2, Lyn, PKA, Syk?and FGR respectively). Exhibits antioxidant, antitumor and anticarcinogenic activity and also inhibits glutat |
Ascorbic acid |
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A8158-10 | ApexBio | 10 mg | EUR 170.4 |
Description: Ascorbic acid is the recommended reagent for the reduction of Copper(II)-TBTA complex into active Copper(I) form. |
Nordihydroguaiaretic acid |
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A3664-100 | ApexBio | 100 mg | EUR 126 |
Description: Description: IC50 Value: N/A Nordihydroguaiaretic acid (NDGA) is a natural phenolic compound isolated from the creosote bush Larrea divaricata, which has anti-tumor activities both in vitro and in vivo. |
Nordihydroguaiaretic acid |
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A3664-500 | ApexBio | 500 mg | EUR 195.6 |
Description: Description: IC50 Value: N/A Nordihydroguaiaretic acid (NDGA) is a natural phenolic compound isolated from the creosote bush Larrea divaricata, which has anti-tumor activities both in vitro and in vivo. |
Oseltamivir acid |
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A3689-10 | ApexBio | 10 mg | EUR 172.8 |
Description: Oseltamivir is an inhibitor of influenza neuraminidase [1].Oseltamivir is a prodrug that is converted by intestinal and/or hepatic esterases to the neuraminidase inhibitor molecule, oseltamivir carboxylate. |
Oseltamivir acid |
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A3689-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 129.6 |
Description: Oseltamivir is an inhibitor of influenza neuraminidase [1].Oseltamivir is a prodrug that is converted by intestinal and/or hepatic esterases to the neuraminidase inhibitor molecule, oseltamivir carboxylate. |
Oseltamivir acid |
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A3689-50 | ApexBio | 50 mg | EUR 315.6 |
Description: Oseltamivir is an inhibitor of influenza neuraminidase [1].Oseltamivir is a prodrug that is converted by intestinal and/or hepatic esterases to the neuraminidase inhibitor molecule, oseltamivir carboxylate. |
Glycerophosphoric acid |
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20-abx180002 | Abbexa |
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Cyclobutylboronic Acid |
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20-abx180715 | Abbexa |
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Hendecanoic acid |
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20-abx180775 | Abbexa |
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Heptadecanoic acid |
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20-abx180776 | Abbexa |
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Methanesulfonylacetic Acid |
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20-abx180797 | Abbexa |
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Octadecanedioic acid |
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20-abx180854 | Abbexa |
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pentadecanoic acid |
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20-abx180857 | Abbexa |
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Pyromeconic acid |
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20-abx180870 | Abbexa |
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Tridecanoic acid |
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20-abx180912 | Abbexa |
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Cyclopentylboronic Acid |
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20-abx181255 | Abbexa |
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Cyclopropylacetic acid |
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20-abx181256 | Abbexa |
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Hydroxymalonic acid |
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20-abx181299 | Abbexa |
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Tridecanedioic acid |
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20-abx181373 | Abbexa |
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Coumalic acid |
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20-abx182402 | Abbexa |
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Cyclohexylboronic Acid |
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20-abx182406 | Abbexa |
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Ethylboronic Acid |
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20-abx182474 | Abbexa |
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Isopropylboronic Acid |
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20-abx182498 | Abbexa |
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Methylboronic Acid |
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20-abx182574 | Abbexa |
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oxalic acid |
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20-abx182613 | Abbexa |
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Chenodeoxycholic acid |
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abx183934-100g | Abbexa | 100 g | EUR 276 |
Identification of the RNase-binding site of SARS-CoV-2 RNA for anchor primer-PCR detection of viral loading in 306 COVID-19 patients
The pandemic of coronavirus disease 2019 (COVID-19) urgently calls for more sensitive molecular diagnosis to improve sensitivity of current viral nuclear acid detection. We have developed an anchor primer (AP)-based assay to improve viral RNA stability by bioinformatics identification of RNase-binding site of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA and implementing AP dually targeting the N gene of SARS-CoV-2 RNA and RNase 1, 3, 6.
The arbitrarily primed polymerase chain reaction (AP-PCR) improvement of viral RNA integrity was supported by (a) the AP increased resistance of the targeted gene (N gene) of SARS-CoV-2 RNA to RNase treatment; (b) the detection of SARS-CoV-2 RNA by AP-PCR with lower cycle threshold values (-2.7 cycles) compared to two commercially available assays; (c) improvement of the viral RNA stability of the ORF gene upon targeting of the N gene and RNase. Furthermore, the improved sensitivity by AP-PCR was demonstrated by detection of SARS-CoV-2 RNA in 70-80% of sputum, nasal, pharyngeal swabs and feces and 36% (4/11) of urine of the confirmed cases (n = 252), 7% convalescent cases (n = 54) and none of 300 negative cases. Lastly, AP-PCR analysis of 306 confirmed and convalescent cases revealed prolonged presence of viral loading for >20 days after the first positive diagnosis.
Thus, the AP dually targeting SARS-CoV-2 RNA and RNase improves molecular detection by preserving SARS-CoV-2 RNA integrity and reveals the prolonged viral loading associated with older age and male gender in COVID-19 patients.
Rapid and Efficient Colony-PCR for High Throughput Screening of Genetically Transformed Chlamydomonas reinhardtii
Microalgae biotechnologies are rapidly developing into new commercial settings. Several high value products already exist on the market, and biotechnological development is focused on genetic engineering of microalgae to open up future economic opportunities for food, fuel and pharmacological production. Colony-polymerase chain reaction (colony-PCR or cPCR) is a critical method for screening genetically transformed microalgae cells.
However, the ability to rapidly screen thousands of transformants using the current colony-PCR method, becomes a very laborious and time-consuming process. Herein, the non-homologous transformation of Chlamydomonas reinhardtii using the electroporation and glass beads methods generated more than seven thousand transformants. In order to manage this impressive number of clones efficiently, we developed a high-throughput screening (HTS) cPCR method to rapidly maximize the detection and selection of positively transformed clones.
For this, we optimized the Chlamydomonas transformed cell layout on the culture media to improve genomic DNA extraction and cPCR in 96-well plate. The application of this optimized HTS cPCR method offers a rapid, less expensive and reliable method for the detection and selection of microalgae transformants.
Our method, which saves up to 80% of the experimental time, holds promise for evaluating genetically transformed cells and selection for microalgae-based biotechnological applications such as synthetic biology and metabolic engineering.
ART5 antibody |
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70R-5344 | Fitzgerald | 50 ug | EUR 560.4 |
Description: Rabbit polyclonal ART5 antibody raised against the middle region of ART5 |
ART5 antibody |
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70R-15855 | Fitzgerald | 50 ul | EUR 522 |
Description: Rabbit polyclonal ART5 antibody |
ART5 Antibody |
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36155-100ul | SAB | 100ul | EUR 302.4 |
Art5/ Rat Art5 ELISA Kit |
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ELI-20661r | Lifescience Market | 96 Tests | EUR 1063.2 |
ART5 Conjugated Antibody |
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C36155 | SAB | 100ul | EUR 476.4 |
anti- ART5 antibody |
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FNab00615 | FN Test | 100µg | EUR 658.5 |
Description: Antibody raised against ART5 |
Anti-ART5 antibody |
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PAab00615 | Lifescience Market | 100 ug | EUR 463.2 |
Anti-ART5 antibody |
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STJ29226 | St John's Laboratory | 100 µl | EUR 332.4 |
Description: The protein encoded by this gene belongs to the ARG-specific ADP-ribosyltransferase family. Proteins in this family regulate the function of target proteins by attaching ADP-ribose to specific amino acid residues in their target proteins. The mouse homolog lacks a glycosylphosphatidylinositol-anchor signal sequence and is predicted to be a secretory enzyme. Several transcripts encoding different isoforms have been found for this gene. |
ART5 Polyclonal Antibody |
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A66466-020 | EpiGentek | 20 ul | EUR 117.7 |
ART5 Polyclonal Antibody |
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A66466-050 | EpiGentek | 50 ul | EUR 302.5 |
ART5 Polyclonal Antibody |
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A66466-100 | EpiGentek | 100 ul | EUR 423.5 |
ART5 Polyclonal Antibody |
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A72754-050 | EpiGentek | 50 ul | EUR 302.5 |
ART5 Polyclonal Antibody |
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A72754-100 | EpiGentek | 100 ul | EUR 423.5 |
ART5 Polyclonal Antibody |
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A66466 | EpiGentek |
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ART5 Polyclonal Antibody |
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A72754 | EpiGentek |
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ART5 siRNA |
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20-abx908254 | Abbexa |
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ART5 siRNA |
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20-abx908255 | Abbexa |
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ART5 siRNA |
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20-abx900464 | Abbexa |
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anti-ART5 |
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YF-PA21994 | Abfrontier | 50 ul | EUR 435.6 |
Description: Mouse polyclonal to ART5 |
anti-ART5 |
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YF-PA21995 | Abfrontier | 50 ug | EUR 435.6 |
Description: Mouse polyclonal to ART5 |
anti-ART5 |
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YF-PA21996 | Abfrontier | 50 ul | EUR 435.6 |
Description: Mouse polyclonal to ART5 |
anti-ART5 |
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YF-PA21997 | Abfrontier | 50 ug | EUR 435.6 |
Description: Mouse polyclonal to ART5 |
ART5 Antibody, HRP conjugated |
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1-CSB-PA836246LB01HU | Cusabio |
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Description: A polyclonal antibody against ART5. Recognizes ART5 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA |
ART5 Antibody, FITC conjugated |
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1-CSB-PA836246LC01HU | Cusabio |
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Description: A polyclonal antibody against ART5. Recognizes ART5 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA |
ART5 Antibody, Biotin conjugated |
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1-CSB-PA836246LD01HU | Cusabio |
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Description: A polyclonal antibody against ART5. Recognizes ART5 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA |
ART5 Rabbit pAb |
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A7146-100ul | Abclonal | 100 ul | EUR 369.6 |
ART5 Rabbit pAb |
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A7146-200ul | Abclonal | 200 ul | EUR 550.8 |
ART5 Rabbit pAb |
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A7146-20ul | Abclonal | 20 ul | EUR 219.6 |
ART5 Rabbit pAb |
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A7146-50ul | Abclonal | 50 ul | EUR 267.6 |
ART5 cloning plasmid |
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CSB-CL836246HU-10ug | Cusabio | 10ug | EUR 279.6 |
Description: A cloning plasmid for the ART5 gene. |
ART5 Blocking Peptide |
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33R-9536 | Fitzgerald | 100 ug | EUR 216 |
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of ART5 antibody, catalog no. 70R-5344 |
ADP-Ribosyltransferase 5 (ART5) Antibody |
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20-abx110890 | Abbexa |
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ADP-Ribosyltransferase 5 (ART5) Antibody |
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20-abx006905 | Abbexa |
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ADP-Ribosyltransferase 5 (ART5) Antibody |
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abx030620-400ul | Abbexa | 400 ul | EUR 627.6 |
ADP-Ribosyltransferase 5 (ART5) Antibody |
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abx030620-80l | Abbexa | 80 µl | EUR 343.2 |
ADP-Ribosyltransferase 5 (ART5) Antibody |
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20-abx212387 | Abbexa |
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ADP-Ribosyltransferase 5 (ART5) Antibody |
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20-abx212388 | Abbexa |
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ART5 Polyclonal Antibody, HRP Conjugated |
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A66467-050 | EpiGentek | 50 ul | EUR 302.5 |
ART5 Polyclonal Antibody, HRP Conjugated |
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A66467-100 | EpiGentek | 100 ul | EUR 423.5 |
ART5 Polyclonal Antibody, FITC Conjugated |
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A66468-050 | EpiGentek | 50 ul | EUR 302.5 |
ART5 Polyclonal Antibody, FITC Conjugated |
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A66468-100 | EpiGentek | 100 ul | EUR 423.5 |
ART5 Polyclonal Antibody, Biotin Conjugated |
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A66469-050 | EpiGentek | 50 ul | EUR 302.5 |
ART5 Polyclonal Antibody, Biotin Conjugated |
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A66469-100 | EpiGentek | 100 ul | EUR 423.5 |
ART5 Polyclonal Antibody, HRP Conjugated |
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A66467 | EpiGentek |
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ART5 Polyclonal Antibody, FITC Conjugated |
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A66468 | EpiGentek |
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ART5 Polyclonal Antibody, Biotin Conjugated |
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A66469 | EpiGentek |
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Mouse ART5 shRNA Plasmid |
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20-abx969241 | Abbexa |
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Rat ART5 shRNA Plasmid |
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20-abx988042 | Abbexa |
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Human ART5 shRNA Plasmid |
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20-abx964539 | Abbexa |
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ART5 ELISA KIT|Human |
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EF007924 | Lifescience Market | 96 Tests | EUR 826.8 |
ART5 Recombinant Protein (Human) |
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RP001990 | ABM | 100 ug | Ask for price |
ART5 Recombinant Protein (Mouse) |
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RP117383 | ABM | 100 ug | Ask for price |
ART5 Recombinant Protein (Rat) |
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RP191087 | ABM | 100 ug | Ask for price |
Ecto-ADP-Ribosyltransferase 5 (ART5) Antibody |
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abx230615-100ug | Abbexa | 100 ug | EUR 610.8 |
Ecto-ADP-Ribosyltransferase 5 (ART5) Antibody |
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20-abx302484 | Abbexa |
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Art5 ORF Vector (Rat) (pORF) |
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ORF063697 | ABM | 1.0 ug DNA | EUR 607.2 |
ART5 ELISA Kit (Human) (OKEH07850) |
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OKEH07850 | Aviva Systems Biology | 96 Wells | EUR 1075.2 |
Description: Description of target: The protein encoded by this gene belongs to the ARG-specific ADP-ribosyltransferase family. Proteins in this family regulate the function of target proteins by attaching ADP-ribose to specific amino acid residues in their target proteins. The mouse homolog lacks a glycosylphosphatidylinositol-anchor signal sequence and is predicted to be a secretory enzyme. Several transcripts encoding different isoforms have been found for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 1.63ng/mL |
ART5 ORF Vector (Human) (pORF) |
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ORF000664 | ABM | 1.0 ug DNA | EUR 114 |
Art5 ORF Vector (Mouse) (pORF) |
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ORF039129 | ABM | 1.0 ug DNA | EUR 607.2 |
Ecto-ADP-Ribosyltransferase 5 (ART5) Antibody (HRP) |
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20-abx309159 | Abbexa |
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Ecto-ADP-Ribosyltransferase 5 (ART5) Antibody (FITC) |
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20-abx309160 | Abbexa |
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Ecto-ADP-Ribosyltransferase 5 (ART5) Antibody (Biotin) |
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20-abx309161 | Abbexa |
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ART5 sgRNA CRISPR Lentivector set (Human) |
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K0129401 | ABM | 3 x 1.0 ug | EUR 406.8 |
Art5 sgRNA CRISPR Lentivector set (Mouse) |
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K4792201 | ABM | 3 x 1.0 ug | EUR 406.8 |
Art5 sgRNA CRISPR Lentivector set (Rat) |
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K7389301 | ABM | 3 x 1.0 ug | EUR 406.8 |
Human ADP-Ribosyltransferase 5 (ART5) ELISA Kit |
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20-abx385881 | Abbexa |
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ART5 sgRNA CRISPR Lentivector (Human) (Target 1) |
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K0129402 | ABM | 1.0 ug DNA | EUR 184.8 |
ART5 sgRNA CRISPR Lentivector (Human) (Target 2) |
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K0129403 | ABM | 1.0 ug DNA | EUR 184.8 |
ART5 sgRNA CRISPR Lentivector (Human) (Target 3) |
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K0129404 | ABM | 1.0 ug DNA | EUR 184.8 |
Art5 sgRNA CRISPR Lentivector (Mouse) (Target 1) |
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K4792202 | ABM | 1.0 ug DNA | EUR 184.8 |
Art5 sgRNA CRISPR Lentivector (Mouse) (Target 2) |
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K4792203 | ABM | 1.0 ug DNA | EUR 184.8 |
Art5 sgRNA CRISPR Lentivector (Mouse) (Target 3) |
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K4792204 | ABM | 1.0 ug DNA | EUR 184.8 |
Art5 sgRNA CRISPR Lentivector (Rat) (Target 1) |
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K7389302 | ABM | 1.0 ug DNA | EUR 184.8 |
Art5 sgRNA CRISPR Lentivector (Rat) (Target 2) |
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K7389303 | ABM | 1.0 ug DNA | EUR 184.8 |
Art5 sgRNA CRISPR Lentivector (Rat) (Target 3) |
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K7389304 | ABM | 1.0 ug DNA | EUR 184.8 |
ART5 Protein Vector (Human) (pPB-C-His) |
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PV002653 | ABM | 500 ng | EUR 394.8 |
ART5 Protein Vector (Human) (pPB-N-His) |
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PV002654 | ABM | 500 ng | EUR 394.8 |
ART5 Protein Vector (Human) (pPM-C-HA) |
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PV002655 | ABM | 500 ng | EUR 394.8 |
ART5 Protein Vector (Human) (pPM-C-His) |
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PV002656 | ABM | 500 ng | EUR 394.8 |
ART5 Protein Vector (Mouse) (pPB-C-His) |
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PV156514 | ABM | 500 ng | EUR 723.6 |
ART5 Protein Vector (Mouse) (pPB-N-His) |
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PV156515 | ABM | 500 ng | EUR 723.6 |
ART5 Protein Vector (Mouse) (pPM-C-HA) |
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PV156516 | ABM | 500 ng | EUR 723.6 |
ART5 Protein Vector (Mouse) (pPM-C-His) |
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PV156517 | ABM | 500 ng | EUR 723.6 |
Human ADP Ribosyltransferase 5(ART5)ELISA Kit |
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QY-E05168 | Qayee Biotechnology | 96T | EUR 480 |
ART5 Protein Vector (Human) (pPB-His-MBP) |
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PV324090 | ABM | 500 ng | EUR 394.8 |
ART5 Protein Vector (Human) (pPB-His-GST) |
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PV324091 | ABM | 500 ng | EUR 394.8 |
ART5 Protein Vector (Rat) (pPB-C-His) |
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PV254786 | ABM | 500 ng | EUR 723.6 |
ART5 Protein Vector (Rat) (pPB-N-His) |
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PV254787 | ABM | 500 ng | EUR 723.6 |
ART5 Protein Vector (Rat) (pPM-C-HA) |
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PV254788 | ABM | 500 ng | EUR 723.6 |
ART5 Protein Vector (Rat) (pPM-C-His) |
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PV254789 | ABM | 500 ng | EUR 723.6 |
ART5 3'UTR Luciferase Stable Cell Line |
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TU001209 | ABM | 1.0 ml | EUR 1672.8 |
Art5 3'UTR Luciferase Stable Cell Line |
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TU200944 | ABM | 1.0 ml | Ask for price |
Art5 3'UTR GFP Stable Cell Line |
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TU250944 | ABM | 1.0 ml | Ask for price |
Art5 3'UTR GFP Stable Cell Line |
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TU152174 | ABM | 1.0 ml | Ask for price |
Art5 3'UTR Luciferase Stable Cell Line |
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TU102174 | ABM | 1.0 ml | Ask for price |
ART5 3'UTR GFP Stable Cell Line |
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TU051209 | ABM | 1.0 ml | EUR 1672.8 |
ART5 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV) |
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LV656155 | ABM | 1.0 ug DNA | EUR 616.8 |
ART5 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC) |
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LV656159 | ABM | 1.0 ug DNA | EUR 616.8 |
ART5 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a) |
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LV656160 | ABM | 1.0 ug DNA | EUR 616.8 |
Human Ecto- ADP- ribosyltransferase 5, ART5 ELISA KIT |
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ELI-44798h | Lifescience Market | 96 Tests | EUR 988.8 |
Mouse Ecto- ADP- ribosyltransferase 5, Art5 ELISA KIT |
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ELI-44883m | Lifescience Market | 96 Tests | EUR 1038 |
Bovine Ecto- ADP- ribosyltransferase 5, ART5 ELISA KIT |
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ELI-20660b | Lifescience Market | 96 Tests | EUR 1113.6 |
ART5 Protein Vector (Human) (pPM-N-D-C-HA) |
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PV324092 | ABM | 500 ng | EUR 662.4 |
ART5 Protein Vector (Human) (pPM-N-D-C-His) |
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PV324093 | ABM | 500 ng | EUR 662.4 |
ART5 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human) |
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K0129405 | ABM | 3 x 1.0 ug | EUR 451.2 |
Art5 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse) |
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K4792205 | ABM | 3 x 1.0 ug | EUR 451.2 |
Art5 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat) |
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K7389305 | ABM | 3 x 1.0 ug | EUR 451.2 |
ART5 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1) |
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K0129406 | ABM | 1.0 ug DNA | EUR 200.4 |
ART5 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2) |
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K0129407 | ABM | 1.0 ug DNA | EUR 200.4 |
ART5 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3) |
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K0129408 | ABM | 1.0 ug DNA | EUR 200.4 |
ART5 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA) |
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LV656156 | ABM | 1.0 ug DNA | EUR 616.8 |