oktober 2, 2022

Infectious Agents Identified by Real-Time PCR

lembcke.dk

Infectious Agents Identified by Real-Time PCR, Serology and Bacteriology in Blood and Peritoneal Exudate Samples of Cows Affected by Parietal Fibrinous Peritonitis after Caesarean Section

The aim of this study was to identify the pathogens potentially involved in parietal fibrinous peritonitis (PFP). PFP is a complication of laparotomy in cattle, characterized by an accumulation of exudate inside a fibrinous capsule. We have studied 72 cases of PFP in Belgian blue cows, confirmed by a standard diagnostic protocol. Blood was collected to evaluate the presence of antibodies for Mycoplasma bovis(M. bovis)Coxiella burnetii(C. burnetii) and Bovine Herpesvirus 4(BoHV4) by enzyme-linked immunosorbent assays.
Peritoneal exudate was obtained from the PFP cavity to perform bacteriological culture, and to identify the DNA of MbovisCburnetii and BoHV4 using real time polymerase chain reaction (qPCR). Bacteriological culture was positive in most peritoneal samples (59/72); Trueperella pyogenes (T. pyogenes) (51/72) and Escherichia coli (E. coli) (20/72) were the most frequently identified. For BoHV4, the majority of cows showed positive serology and qPCR (56/72 and 49/72, respectively).
Contrariwise, M. bovis (17/72 and 6/72, respectively) and C. burnetii (15/72 and 6/72, respectively) were less frequently detected (p < 0.0001). Our study proves that PFP can no longer be qualified as a sterile inflammation. Moreover, we herein describe the first identification of BoHV4 and C. burnetii in cows affected by PFP.

lembcke.dk

Valproic Acid

M41002 500 mg
EUR 128.3
Description: The best epigenetics products

Ginkgolic acid

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Description: kits suitable for this type of research

Mucic acid

MD1225 50g
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Nalidixic Acid

N011-100G 100 g
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Nalidixic Acid

N011-25G 25 g
EUR 77

Neochlorogenic acid

N050-10MG 10 mg
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Neochlorogenic acid

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EUR 617

Oxolinic acid

O002-25G 25 g
EUR 335

Oxolinic acid

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Lactobionic acid

L017-25G 25 g
EUR 99

Mycophenolic Acid

M012-250MG 250 mg
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Mycophenolic Acid

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Pipemidic acid

P009-100G 100 g
EUR 411

Pipemidic acid

P009-10G 10 g
EUR 91

Pseudomonic acid

P095-100MG 100 mg
EUR 708

Pseudomonic acid

P095-25MG 25 mg
EUR 224

Zoledronic Acid

HY-13777 100mg
EUR 229

Retinoic acid

HY-14649 10mM/1mL
EUR 126

Levomefolic acid

HY-14781 10mM/1mL
EUR 231

Minodronic acid

HY-16322 10mg
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Folic acid

HY-16637 500mg
EUR 108

Pirinixic acid

HY-16995 250mg
EUR 354

Folinic acid

HY-17556 100mg
EUR 667

Lipoic acid

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EUR 133

Ethylmalonic acid

HY-34740 500mg
EUR 108

Octanoic acid

HY-41417 5g
EUR 119

(-)-Aspartic acid

HY-42068 5g
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Asparagusic acid

HY-50730 100mg
EUR 609

Chenodeoxycholic Acid

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Glyoxalic acid

HY-79494 500mg
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Benzenebutyric acid

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Pamidronic acid

HY-B0012 10mM/1mL
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Risedronic acid

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EUR 402

Tranexamic acid

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Salicylic acid

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Ellagic acid

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Etidronic acid

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Tolfenamic Acid

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Nalidixic acid

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Mycophenolic acid

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Niflumic acid

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Valproic acid

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Arundic Acid

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Medronic acid

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Aminomalonic acid

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Urocanic acid

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Hydroxypyruvic acid

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Mevalonic acid

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Argininic acid

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Paullinic acid

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Indolelactic acid

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Eicosadienoic acid

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Bovinic acid

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Ursocholic acid

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Hydroxyphenyllactic acid

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Oxoadipic acid

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Dihydrofolic acid

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Homogentisic acid

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Ureidopropionic acid

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Citraconic acid

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Hexacosanoic acid

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Aminoadipic acid

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Nicotinuric acid

HY-113353 10mM/1mL
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Petroselinic acid

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Guanidinosuccinic acid

HY-113373 100mg
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Imidazoleacetic acid

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Obeticholic acid

HY-12222 100mg
EUR 383

Bempedoic acid

HY-12357 100mg
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Eicosatetraynoic acid

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Pentacosanoic acid

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Glycodeoxycholic Acid

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Phosphonoacetic acid

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Oseltamivir (acid)

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Fusidic acid

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Fusidic acid

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Benzophenonetetracarboxylic acid

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Kynurenic acid

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Quinolinic acid

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Spaglumic Acid

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Docosatrienoic Acid

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Vanilpyruvic acid

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Bucloxic acid

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Methylmalonic acid

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Fuchsin acid

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Gallic acid

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Chlorogenic acid

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Ferulic acid

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Ginkgolic Acid

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Gambogic Acid

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Shikimic acid

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Ursolic acid

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Oleanolic Acid

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Orotic acid

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Hyodeoxycholic acid

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Caffeic acid

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Glycyrrhizic acid

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Asiatic acid

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Nordihydroguaiaretic acid

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Benzoic acid

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Echinocystic acid

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Corosolic acid

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Protocatechuic acid

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Caftaric acid

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Cholic acid

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Syringic acid

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Pachymic acid

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Identification of the RNase-binding site of SARS-CoV-2 RNA for anchor primer-PCR detection of viral loading in 306 COVID-19 patients

The pandemic of coronavirus disease 2019 (COVID-19) urgently calls for more sensitive molecular diagnosis to improve sensitivity of current viral nuclear acid detection. We have developed an anchor primer (AP)-based assay to improve viral RNA stability by bioinformatics identification of RNase-binding site of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA and implementing AP dually targeting the N gene of SARS-CoV-2 RNA and RNase 1, 3, 6.
The arbitrarily primed polymerase chain reaction (AP-PCR) improvement of viral RNA integrity was supported by (a) the AP increased resistance of the targeted gene (N gene) of SARS-CoV-2 RNA to RNase treatment; (b) the detection of SARS-CoV-2 RNA by AP-PCR with lower cycle threshold values (-2.7 cycles) compared to two commercially available assays; (c) improvement of the viral RNA stability of the ORF gene upon targeting of the N gene and RNase. Furthermore, the improved sensitivity by AP-PCR was demonstrated by detection of SARS-CoV-2 RNA in 70-80% of sputum, nasal, pharyngeal swabs and feces and 36% (4/11) of urine of the confirmed cases (n = 252), 7% convalescent cases (n = 54) and none of 300 negative cases. Lastly, AP-PCR analysis of 306 confirmed and convalescent cases revealed prolonged presence of viral loading for >20 days after the first positive diagnosis.
Thus, the AP dually targeting SARS-CoV-2 RNA and RNase improves molecular detection by preserving SARS-CoV-2 RNA integrity and reveals the prolonged viral loading associated with older age and male gender in COVID-19 patients.

Rapid and Efficient Colony-PCR for High Throughput Screening of Genetically Transformed Chlamydomonas reinhardtii

Microalgae biotechnologies are rapidly developing into new commercial settings. Several high value products already exist on the market, and biotechnological development is focused on genetic engineering of microalgae to open up future economic opportunities for food, fuel and pharmacological production. Colony-polymerase chain reaction (colony-PCR or cPCR) is a critical method for screening genetically transformed microalgae cells.
However, the ability to rapidly screen thousands of transformants using the current colony-PCR method, becomes a very laborious and time-consuming process. Herein, the non-homologous transformation of Chlamydomonas reinhardtii using the electroporation and glass beads methods generated more than seven thousand transformants. In order to manage this impressive number of clones efficiently, we developed a high-throughput screening (HTS) cPCR method to rapidly maximize the detection and selection of positively transformed clones.
For this, we optimized the Chlamydomonas transformed cell layout on the culture media to improve genomic DNA extraction and cPCR in 96-well plate. The application of this optimized HTS cPCR method offers a rapid, less expensive and reliable method for the detection and selection of microalgae transformants.
Our method, which saves up to 80% of the experimental time, holds promise for evaluating genetically transformed cells and selection for microalgae-based biotechnological applications such as synthetic biology and metabolic engineering.

ART5 Antibody

36155-100ul 100ul
EUR 252

ART5 antibody

70R-15855 50 ul
EUR 435
Description: Rabbit polyclonal ART5 antibody

ART5 antibody

70R-5344 50 ug
EUR 467
Description: Rabbit polyclonal ART5 antibody raised against the middle region of ART5

Art5/ Rat Art5 ELISA Kit

ELI-20661r 96 Tests
EUR 886

Anti-ART5 antibody

STJ29226 100 µl
EUR 277
Description: The protein encoded by this gene belongs to the ARG-specific ADP-ribosyltransferase family. Proteins in this family regulate the function of target proteins by attaching ADP-ribose to specific amino acid residues in their target proteins. The mouse homolog lacks a glycosylphosphatidylinositol-anchor signal sequence and is predicted to be a secretory enzyme. Several transcripts encoding different isoforms have been found for this gene.

ART5 Polyclonal Antibody

A66466 100 µg
EUR 570.55
Description: fast delivery possible

anti- ART5 antibody

FNab00615 100µg
EUR 548.75
Description: Antibody raised against ART5

ART5 Conjugated Antibody

C36155 100ul
EUR 397

Anti-ART5 antibody

PAab00615 100 ug
EUR 386

ART5 siRNA

20-abx908254
  • EUR 551.00
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  • 15 nmol
  • 30 nmol

ART5 siRNA

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  • EUR 551.00
  • EUR 732.00
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ART5 siRNA

20-abx900464
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

anti-ART5

YF-PA21994 50 ul
EUR 363
Description: Mouse polyclonal to ART5

anti-ART5

YF-PA21995 50 ug
EUR 363
Description: Mouse polyclonal to ART5

anti-ART5

YF-PA21996 50 ul
EUR 363
Description: Mouse polyclonal to ART5

anti-ART5

YF-PA21997 50 ug
EUR 363
Description: Mouse polyclonal to ART5

ART5 Antibody, HRP conjugated

1-CSB-PA836246LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ART5. Recognizes ART5 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

ART5 Antibody, FITC conjugated

1-CSB-PA836246LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ART5. Recognizes ART5 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

ART5 Antibody, Biotin conjugated

1-CSB-PA836246LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against ART5. Recognizes ART5 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

ART5 cloning plasmid

CSB-CL836246HU-10ug 10ug
EUR 233
Description: A cloning plasmid for the ART5 gene.

ART5 Rabbit pAb

A7146-100ul 100 ul
EUR 308

ART5 Rabbit pAb

A7146-200ul 200 ul
EUR 459

ART5 Rabbit pAb

A7146-20ul 20 ul
EUR 183

ART5 Rabbit pAb

A7146-50ul 50 ul
EUR 223

ART5 Blocking Peptide

33R-9536 100 ug
EUR 180
Description: A synthetic peptide for use as a blocking control in assays to test for specificity of ART5 antibody, catalog no. 70R-5344

ADP-Ribosyltransferase 5 (ART5) Antibody

20-abx110890
  • EUR 732.00
  • EUR 398.00
  • 150 ul
  • 50 ul

ADP-Ribosyltransferase 5 (ART5) Antibody

20-abx006905
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul

ADP-Ribosyltransferase 5 (ART5) Antibody

abx030620-400ul 400 ul
EUR 523

ADP-Ribosyltransferase 5 (ART5) Antibody

abx030620-80l 80 µl
EUR 286

ART5 Polyclonal Antibody, HRP Conjugated

A66467 100 µg
EUR 570.55
Description: reagents widely cited

ART5 Polyclonal Antibody, FITC Conjugated

A66468 100 µg
EUR 570.55
Description: Ask the seller for details

ART5 Polyclonal Antibody, Biotin Conjugated

A66469 100 µg
EUR 570.55
Description: The best epigenetics products

ADP-Ribosyltransferase 5 (ART5) Antibody

20-abx212387
  • EUR 411.00
  • EUR 300.00
  • 100 ul
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ADP-Ribosyltransferase 5 (ART5) Antibody

20-abx212388
  • EUR 411.00
  • EUR 300.00
  • 100 ul
  • 50 ul

Mouse ART5 shRNA Plasmid

20-abx969241
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

Human ART5 shRNA Plasmid

20-abx964539
  • EUR 801.00
  • EUR 1121.00
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  • 300 µg

Rat ART5 shRNA Plasmid

20-abx988042
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

ART5 ELISA KIT|Human

EF007924 96 Tests
EUR 689

ART5 Recombinant Protein (Mouse)

RP117383 100 ug Ask for price

ART5 Recombinant Protein (Rat)

RP191087 100 ug Ask for price

ART5 Recombinant Protein (Human)

RP001990 100 ug Ask for price

Ecto-ADP-Ribosyltransferase 5 (ART5) Antibody

20-abx302484
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
  • 20 ug
  • 50 ug

Ecto-ADP-Ribosyltransferase 5 (ART5) Antibody

abx230615-100ug 100 ug
EUR 509

Ecto-ADP-Ribosyltransferase 5 (ART5) Antibody (HRP)

20-abx309159
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
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Ecto-ADP-Ribosyltransferase 5 (ART5) Antibody (FITC)

20-abx309160
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
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Ecto-ADP-Ribosyltransferase 5 (ART5) Antibody (Biotin)

20-abx309161
  • EUR 411.00
  • EUR 1845.00
  • EUR 599.00
  • EUR 182.00
  • EUR 300.00
  • 100 ug
  • 1 mg
  • 200 ug
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ART5 ELISA Kit (Human) (OKEH07850)

OKEH07850 96 Wells
EUR 896
Description: Description of target: The protein encoded by this gene belongs to the ARG-specific ADP-ribosyltransferase family. Proteins in this family regulate the function of target proteins by attaching ADP-ribose to specific amino acid residues in their target proteins. The mouse homolog lacks a glycosylphosphatidylinositol-anchor signal sequence and is predicted to be a secretory enzyme. Several transcripts encoding different isoforms have been found for this gene.;Species reactivity: Human;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 1.63ng/mL

ART5 ORF Vector (Human) (pORF)

ORF000664 1.0 ug DNA
EUR 95

Art5 ORF Vector (Mouse) (pORF)

ORF039129 1.0 ug DNA
EUR 506

Art5 ORF Vector (Rat) (pORF)

ORF063697 1.0 ug DNA
EUR 506

ART5 sgRNA CRISPR Lentivector set (Human)

K0129401 3 x 1.0 ug
EUR 339

Art5 sgRNA CRISPR Lentivector set (Rat)

K7389301 3 x 1.0 ug
EUR 339

Art5 sgRNA CRISPR Lentivector set (Mouse)

K4792201 3 x 1.0 ug
EUR 339

Human ADP-Ribosyltransferase 5 (ART5) ELISA Kit

20-abx385881
  • EUR 7378.00
  • EUR 3933.00
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  • 10 × 96 tests
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  • 96 tests

Human ADP Ribosyltransferase 5(ART5)ELISA Kit

QY-E05168 96T
EUR 400

ART5 sgRNA CRISPR Lentivector (Human) (Target 1)

K0129402 1.0 ug DNA
EUR 154

ART5 sgRNA CRISPR Lentivector (Human) (Target 2)

K0129403 1.0 ug DNA
EUR 154

ART5 sgRNA CRISPR Lentivector (Human) (Target 3)

K0129404 1.0 ug DNA
EUR 154

Art5 sgRNA CRISPR Lentivector (Rat) (Target 1)

K7389302 1.0 ug DNA
EUR 154

Art5 sgRNA CRISPR Lentivector (Rat) (Target 2)

K7389303 1.0 ug DNA
EUR 154

Art5 sgRNA CRISPR Lentivector (Rat) (Target 3)

K7389304 1.0 ug DNA
EUR 154

Art5 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K4792202 1.0 ug DNA
EUR 154

Art5 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K4792203 1.0 ug DNA
EUR 154

Art5 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K4792204 1.0 ug DNA
EUR 154

Art5 3'UTR Luciferase Stable Cell Line

TU102174 1.0 ml Ask for price

ART5 Protein Vector (Mouse) (pPB-C-His)

PV156514 500 ng
EUR 603

ART5 Protein Vector (Mouse) (pPB-N-His)

PV156515 500 ng
EUR 603

ART5 Protein Vector (Mouse) (pPM-C-HA)

PV156516 500 ng
EUR 603

ART5 Protein Vector (Mouse) (pPM-C-His)

PV156517 500 ng
EUR 603

ART5 Protein Vector (Human) (pPB-His-MBP)

PV324090 500 ng
EUR 329

ART5 Protein Vector (Human) (pPB-His-GST)

PV324091 500 ng
EUR 329

ART5 Protein Vector (Rat) (pPB-C-His)

PV254786 500 ng
EUR 603

ART5 Protein Vector (Rat) (pPB-N-His)

PV254787 500 ng
EUR 603

ART5 Protein Vector (Rat) (pPM-C-HA)

PV254788 500 ng
EUR 603

ART5 Protein Vector (Rat) (pPM-C-His)

PV254789 500 ng
EUR 603

ART5 Protein Vector (Human) (pPB-C-His)

PV002653 500 ng
EUR 329

ART5 Protein Vector (Human) (pPB-N-His)

PV002654 500 ng
EUR 329

ART5 Protein Vector (Human) (pPM-C-HA)

PV002655 500 ng
EUR 329

ART5 Protein Vector (Human) (pPM-C-His)

PV002656 500 ng
EUR 329

Art5 3'UTR Luciferase Stable Cell Line

TU200944 1.0 ml Ask for price

Art5 3'UTR GFP Stable Cell Line

TU250944 1.0 ml Ask for price

ART5 3'UTR GFP Stable Cell Line

TU051209 1.0 ml
EUR 1394

Art5 3'UTR GFP Stable Cell Line

TU152174 1.0 ml Ask for price

ART5 3'UTR Luciferase Stable Cell Line

TU001209 1.0 ml
EUR 1394

Human Ecto- ADP- ribosyltransferase 5, ART5 ELISA KIT

ELI-44798h 96 Tests
EUR 824

Mouse Ecto- ADP- ribosyltransferase 5, Art5 ELISA KIT

ELI-44883m 96 Tests
EUR 865

Bovine Ecto- ADP- ribosyltransferase 5, ART5 ELISA KIT

ELI-20660b 96 Tests
EUR 928

ART5 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)

LV656155 1.0 ug DNA
EUR 514

ART5 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)

LV656159 1.0 ug DNA
EUR 514

ART5 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)

LV656160 1.0 ug DNA
EUR 514

ART5 Protein Vector (Human) (pPM-N-D-C-HA)

PV324092 500 ng
EUR 552

ART5 Protein Vector (Human) (pPM-N-D-C-His)

PV324093 500 ng
EUR 552

ART5 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Human)

K0129405 3 x 1.0 ug
EUR 376

Art5 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Rat)

K7389305 3 x 1.0 ug
EUR 376

Art5 sgRNA CRISPR/Cas9 All-in-One Lentivector set (Mouse)

K4792205 3 x 1.0 ug
EUR 376

ART5 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 1)

K0129406 1.0 ug DNA
EUR 167

ART5 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 2)

K0129407 1.0 ug DNA
EUR 167

ART5 sgRNA CRISPR/Cas9 All-in-One Lentivector (Human) (Target 3)

K0129408 1.0 ug DNA
EUR 167

Art5 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 1)

K7389306 1.0 ug DNA
EUR 167

Art5 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 2)

K7389307 1.0 ug DNA
EUR 167

Art5 sgRNA CRISPR/Cas9 All-in-One Lentivector (Rat) (Target 3)

K7389308 1.0 ug DNA
EUR 167

ART5 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-C-term-HA)

LV656156 1.0 ug DNA
EUR 514

ART5 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-GFP-2A-Puro)

LV656157 1.0 ug DNA
EUR 572

ART5 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV-RFP-2A-Puro)

LV656158 1.0 ug DNA
EUR 572

Art5 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 1)

K4792206 1.0 ug DNA
EUR 167

Art5 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 2)

K4792207 1.0 ug DNA
EUR 167

Art5 sgRNA CRISPR/Cas9 All-in-One Lentivector (Mouse) (Target 3)

K4792208 1.0 ug DNA
EUR 167

CD11b Antibody Antibody

ABD2911 100 ug
EUR 438

H2B Antibody Antibody

AF4659 200ul
EUR 376
Description: H2B Antibody Antibody detects endogenous levels of H2B.

anti- Antibody^Polyclonal antibody control antibody

LSMab09882 100 ug
EUR 438

Antibody

A1360-500 Ask for price