PCR-RFLP: a focused methodology to disclose host particular malacosporean an infection profiles (Cnidaria: Myxozoa: Malacosporea)
Malacosporeans are a bunch of endoparasitic cnidarians (Myxozoa) that use freshwater bryozoans and fish as last and intermediate hosts, respectively. The malacosporean Tetracapsuloides bryosalmonae causes proliferative kidney illness (PKD), an rising illness in aquaculture and wild fish populations, together with threatened salmonids in Europe and the USA.
Combined infections of malacosporeans are sometimes encountered, and a monitoring software for screening of a number of malacosporean species in both their fish or bryozoan hosts is due to this fact fascinating. We describe a cheap methodology that mixes PCR amplification of the partial 18S rRNA gene (~260 bp) and a single-step restriction fragment size polymorphism (RFLP) methodology for identification of 10 malacosporean lineages and species.
We display and take a look at this technique on a set of DNA extracted from malacosporeans infecting fish kidney and tissues sampled from bryozoan colonies and examine the outcomes with Sanger sequencing of the identical parasite DNA isolates. The PCR-RFLP and Sanger sequencing strategies agreed in 100% of circumstances. The PCR-RFLP methodology provides a variety of alternatives, together with screening giant panels of host tissue samples to achieve insights into an infection patterns, characterizing combined infections, and confirming extremely pathogenic T. bryosalmonae infections.
The strategy will also be additional refined as new sequence information turn into accessible for malacosporeans.
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human, Mouse, Rat, Monkey. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/40000
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:200-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:10-1:50
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:10-1:50
Description: A polyclonal antibody for detection of TPH2 from Human, Mouse, Rat, Monkey. This TPH2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the non-phosphorylation site of S19
Description: A polyclonal antibody for detection of TPH2 from Human, Mouse, Rat, Monkey. This TPH2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the non-phosphorylation site of S19
Description: A polyclonal antibody for detection of TPH2 from Human, Mouse, Rat, Monkey. This TPH2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the non-phosphorylation site of S19
Description: This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.
Description: This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.
Description: A polyclonal antibody against TPH2 (Ab-19). Recognizes TPH2 (Ab-19) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against TPH2 (Ab-19). Recognizes TPH2 (Ab-19) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against Phospho-TPH2 (S19). Recognizes Phospho-TPH2 (S19) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/40000
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against Phospho-TPH2 (Ser19). Recognizes Phospho-TPH2 (Ser19) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000
Description: A polyclonal antibody against Phospho-TPH2 (Ser19). Recognizes Phospho-TPH2 (Ser19) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000
Description: A polyclonal antibody for detection of TPH2 phospho Ser19) from Human, Mouse, Rat. This TPH2 phospho Ser19) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the phosphorylation site of S19
Description: A polyclonal antibody for detection of TPH2 phospho Ser19) from Human, Mouse, Rat. This TPH2 phospho Ser19) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the phosphorylation site of S19
Description: A polyclonal antibody for detection of TPH2 phospho Ser19) from Human, Mouse, Rat. This TPH2 phospho Ser19) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the phosphorylation site of S19
Description: A Rabbit Polyclonal antibody against TPH2 (phospho Ser19) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: A Rabbit Polyclonal antibody against TPH2 (phospho Ser19) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human TPH2 (aa16-29) (N-Term). This antibody is tested and proven to work in the following applications:
Description: Description of target: Tryptophan hydroxylase (TPH; EC 1.14.16.4) is the rate-limiting enzyme in the synthesis of serotonin (5-hydroxytryptamine, or 5HT). 5HT is causally involved in numerous central nervous activities, and it has several functions in peripheral tissues, including the maintenance of vascular tone and gut motility.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.119ng/mL
Description: Description of target: isoform of tryptophan hydroxylase; may play a role in seratonin biosynthesis [RGD, Feb 2006];Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.56 ng/mL
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Tryptophan hydroxylase 2 / TPH2 (internal region). This antibody is tested and proven to work in the following applications:
Analysis of BOX-PCR and REP-PCR as Molecular Typing Instruments for Antarctic Streptomyces
Molecular research led to the resurgence of pure merchandise analysis from genus Streptomyces, already identified for his or her lengthy historical past and significance for the pharmaceutical trade. Nevertheless, species belonging to this genus are troublesome to establish and probably the most generally used strategies, that are primarily based on 16S rRNA sequencing, don’t discriminate between associated species. On this work, amplification profiles generated from BOX-PCR and REP-PCR of 49 Antarctic soil streptomycetes had been in comparison with consider the variety current within the group and to characterize the bacterial isolates, together with some 16S rRNA amplifications.
The BOX-A1R primer exhibit clearer amplification fragments, totally different from the amplification patterns obtained utilizing the REP 1R and 2R primers. A better variety was noticed with REP-PCR amplifications, regardless that a bigger variety of fragments was obtained with BOX-A1R primer amplifications. There are not less than 4 isolates that confirmed nice similarity (about 90%) in each strategies.
In different hand, there are two others which might be 90% related in BOX-PCR, however distant in REP-PCR, exhibiting solely 40% of similarity. Outcomes of the mixture of BOX-PCR and REP-PCR signify a easy and low-cost methodology to discriminate between Streptomyces strains. There isn’t any species identification with solely the 16S rRNA, most isolates appear to be associated to S. globisporus. Additional research added to the obtained outcomes could present higher information to assist the characterization of those microorganisms.
Digital PCR-based plasma cell-free DNA mutation evaluation for early-stage pancreatic tumor analysis and surveillance
Background: Cell-free DNA (cfDNA) shed from tumors into the circulation provides a software for most cancers detection. Right here, we evaluated the feasibility of cfDNA measurement and utility of digital PCR (dPCR)-based assays, which scale back subsampling error, for diagnosing pancreatic ductal adenocarcinoma (PDA) and surveillance of intraductal papillary mucinous neoplasm (IPMN).
Strategies: We collected plasma from seven establishments for cfDNA measurements. Scorching-spot mutations in KRAS and GNAS within the cfDNA from sufferers with PDA (n = 96), present process surveillance for IPMN (n = 112), and regular controls (n = 76) had been evaluated utilizing pre-amplification dPCR.
Outcomes: Upon Qubit measurement and duplicate quantity evaluation of hemoglobin-subunit (HBB) and mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 1 (MT-ND1) in plasma cfDNA, HBB provided the perfect decision between sufferers with PDA relative to wholesome topics [area under the curve (AUC) 0.862], whereas MT-ND1 revealed vital variations between IPMN and controls (AUC 0.851).
DPCR using pre-amplification cfDNA afforded correct tumor-derived mutant KRAS detection in plasma in resectable PDA (AUC 0.861-0.876) and improved post-resection recurrence prediction [hazard ratio (HR) 3.179, 95% confidence interval (CI) 1.025-9.859] over that for the marker CA19-9 (HR 1.464; 95% CI 0.674-3.181). Capturing KRAS and GNAS may additionally present genetic proof in sufferers with IPMN-associated PDA and present process pancreatic surveillance.
Conclusions: Plasma cfDNA quantification by distinct measurements is helpful to foretell tumor burden. By acceptable strategies, dPCR-mediated mutation detection in sufferers with localized PDA and IPMN prone to progress to invasive carcinoma is possible and enhances typical biomarkers.
Description: A polyclonal antibody against KAT7. Recognizes KAT7 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TUBB3. Recognizes TUBB3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TUBB3. Recognizes TUBB3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TMEM109. Recognizes TMEM109 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against DNAJB1. Recognizes DNAJB1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against virD2. Recognizes virD2 from Agrobacterium tumefaciens. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against RELA. Recognizes RELA from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against AFG3L2. Recognizes AFG3L2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against SNX22. Recognizes SNX22 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against GPR161. Recognizes GPR161 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against SARS. Recognizes SARS from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against PFN1. Recognizes PFN1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TUBA1B. Recognizes TUBA1B from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against SLC6A7. Recognizes SLC6A7 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against RPS14. Recognizes RPS14 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against HNRNPH1. Recognizes HNRNPH1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TALDO1. Recognizes TALDO1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against AKAP17A. Recognizes AKAP17A from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against PTDSS1. Recognizes PTDSS1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against RAC1. Recognizes RAC1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against ABL1. Recognizes ABL1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against GTPBP6. Recognizes GTPBP6 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TAX1BP3. Recognizes TAX1BP3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against FCGR2A. Recognizes FCGR2A from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against JUND. Recognizes JUND from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against EFCAB14. Recognizes EFCAB14 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against CHMP2A. Recognizes CHMP2A from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against RBPMS. Recognizes RBPMS from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against PIN1. Recognizes PIN1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against LASP1. Recognizes LASP1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against UQCRFS1. Recognizes UQCRFS1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against SLC6A8. Recognizes SLC6A8 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against KARS. Recognizes KARS from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against CFL1. Recognizes CFL1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TMED9. Recognizes TMED9 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against MRPL9. Recognizes MRPL9 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against PDCD6. Recognizes PDCD6 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against MAP2K2. Recognizes MAP2K2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against DUOXA1. Recognizes DUOXA1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against COMMD4. Recognizes COMMD4 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against SUMO2. Recognizes SUMO2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against HSPA5. Recognizes HSPA5 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against CNPY2. Recognizes CNPY2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TMED2. Recognizes TMED2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against LYPLA2. Recognizes LYPLA2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against BTBD2. Recognizes BTBD2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against ANAPC5. Recognizes ANAPC5 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against RPN1. Recognizes RPN1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA