PCR-RFLP: a focused methodology to disclose host particular malacosporean an infection profiles (Cnidaria: Myxozoa: Malacosporea)
Malacosporeans are a bunch of endoparasitic cnidarians (Myxozoa) that use freshwater bryozoans and fish as last and intermediate hosts, respectively. The malacosporean Tetracapsuloides bryosalmonae causes proliferative kidney illness (PKD), an rising illness in aquaculture and wild fish populations, together with threatened salmonids in Europe and the USA.
Combined infections of malacosporeans are sometimes encountered, and a monitoring software for screening of a number of malacosporean species in both their fish or bryozoan hosts is due to this fact fascinating. We describe a cheap methodology that mixes PCR amplification of the partial 18S rRNA gene (~260 bp) and a single-step restriction fragment size polymorphism (RFLP) methodology for identification of 10 malacosporean lineages and species.
We display and take a look at this technique on a set of DNA extracted from malacosporeans infecting fish kidney and tissues sampled from bryozoan colonies and examine the outcomes with Sanger sequencing of the identical parasite DNA isolates. The PCR-RFLP and Sanger sequencing strategies agreed in 100% of circumstances. The PCR-RFLP methodology provides a variety of alternatives, together with screening giant panels of host tissue samples to achieve insights into an infection patterns, characterizing combined infections, and confirming extremely pathogenic T. bryosalmonae infections.
The strategy will also be additional refined as new sequence information turn into accessible for malacosporeans.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Tryptophan Hydroxylase 2 (TPH2) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Tryptophan Hydroxylase 2 (TPH2) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human, Mouse, Rat, Monkey. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/40000
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:200-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:10-1:50
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:10-1:50
Description: This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.
Description: This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.
Description: A polyclonal antibody for detection of TPH2 from Human, Mouse, Rat, Monkey. This TPH2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the non-phosphorylation site of S19
Description: A polyclonal antibody for detection of TPH2 from Human, Mouse, Rat, Monkey. This TPH2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the non-phosphorylation site of S19
Description: A polyclonal antibody for detection of TPH2 from Human, Mouse, Rat, Monkey. This TPH2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the non-phosphorylation site of S19
Description: A polyclonal antibody against TPH2 (Ab-19). Recognizes TPH2 (Ab-19) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against TPH2 (Ab-19). Recognizes TPH2 (Ab-19) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against Phospho-TPH2 (S19). Recognizes Phospho-TPH2 (S19) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/40000
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against Phospho-TPH2 (Ser19). Recognizes Phospho-TPH2 (Ser19) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000
Description: A polyclonal antibody against Phospho-TPH2 (Ser19). Recognizes Phospho-TPH2 (Ser19) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000
Description: A Rabbit Polyclonal antibody against TPH2 (phospho Ser19) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: A Rabbit Polyclonal antibody against TPH2 (phospho Ser19) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: A polyclonal antibody for detection of TPH2 phospho Ser19) from Human, Mouse, Rat. This TPH2 phospho Ser19) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the phosphorylation site of S19
Description: A polyclonal antibody for detection of TPH2 phospho Ser19) from Human, Mouse, Rat. This TPH2 phospho Ser19) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the phosphorylation site of S19
Description: A polyclonal antibody for detection of TPH2 phospho Ser19) from Human, Mouse, Rat. This TPH2 phospho Ser19) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the phosphorylation site of S19
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human TPH2 (aa16-29) (N-Term). This antibody is tested and proven to work in the following applications:
Description: Description of target: Tryptophan hydroxylase (TPH; EC 1.14.16.4) is the rate-limiting enzyme in the synthesis of serotonin (5-hydroxytryptamine, or 5HT). 5HT is causally involved in numerous central nervous activities, and it has several functions in peripheral tissues, including the maintenance of vascular tone and gut motility.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.119ng/mL
Description: Description of target: isoform of tryptophan hydroxylase; may play a role in seratonin biosynthesis [RGD, Feb 2006];Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.56 ng/mL
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Tryptophan hydroxylase 2 / TPH2 (internal region). This antibody is tested and proven to work in the following applications:
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tryptophan Hydroxylase 2 (TPH2) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tryptophan Hydroxylase 2 (TPH2) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tryptophan Hydroxylase 2 (TPH2) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Tryptophan Hydroxylase 2 (TPH2) in serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Tryptophan Hydroxylase 2 (TPH2) in samples from Serum, plasma, tissue homogenates and other biological fluids. with no significant corss-reactivity with analogues from other species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tryptophan Hydroxylase 2 (TPH2) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tryptophan Hydroxylase 2 (TPH2) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tryptophan Hydroxylase 2 (TPH2) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Tryptophan Hydroxylase 2 (TPH2) in tissue homogenates, cell lysates and other biological fluids.
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Analysis of BOX-PCR and REP-PCR as Molecular Typing Instruments for Antarctic Streptomyces
Molecular research led to the resurgence of pure merchandise analysis from genus Streptomyces, already identified for his or her lengthy historical past and significance for the pharmaceutical trade. Nevertheless, species belonging to this genus are troublesome to establish and probably the most generally used strategies, that are primarily based on 16S rRNA sequencing, don’t discriminate between associated species. On this work, amplification profiles generated from BOX-PCR and REP-PCR of 49 Antarctic soil streptomycetes had been in comparison with consider the variety current within the group and to characterize the bacterial isolates, together with some 16S rRNA amplifications.
The BOX-A1R primer exhibit clearer amplification fragments, totally different from the amplification patterns obtained utilizing the REP 1R and 2R primers. A better variety was noticed with REP-PCR amplifications, regardless that a bigger variety of fragments was obtained with BOX-A1R primer amplifications. There are not less than 4 isolates that confirmed nice similarity (about 90%) in each strategies.
In different hand, there are two others which might be 90% related in BOX-PCR, however distant in REP-PCR, exhibiting solely 40% of similarity. Outcomes of the mixture of BOX-PCR and REP-PCR signify a easy and low-cost methodology to discriminate between Streptomyces strains. There isn’t any species identification with solely the 16S rRNA, most isolates appear to be associated to S. globisporus. Additional research added to the obtained outcomes could present higher information to assist the characterization of those microorganisms.
Digital PCR-based plasma cell-free DNA mutation evaluation for early-stage pancreatic tumor analysis and surveillance
Background: Cell-free DNA (cfDNA) shed from tumors into the circulation provides a software for most cancers detection. Right here, we evaluated the feasibility of cfDNA measurement and utility of digital PCR (dPCR)-based assays, which scale back subsampling error, for diagnosing pancreatic ductal adenocarcinoma (PDA) and surveillance of intraductal papillary mucinous neoplasm (IPMN).
Strategies: We collected plasma from seven establishments for cfDNA measurements. Scorching-spot mutations in KRAS and GNAS within the cfDNA from sufferers with PDA (n = 96), present process surveillance for IPMN (n = 112), and regular controls (n = 76) had been evaluated utilizing pre-amplification dPCR.
Outcomes: Upon Qubit measurement and duplicate quantity evaluation of hemoglobin-subunit (HBB) and mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 1 (MT-ND1) in plasma cfDNA, HBB provided the perfect decision between sufferers with PDA relative to wholesome topics [area under the curve (AUC) 0.862], whereas MT-ND1 revealed vital variations between IPMN and controls (AUC 0.851).
DPCR using pre-amplification cfDNA afforded correct tumor-derived mutant KRAS detection in plasma in resectable PDA (AUC 0.861-0.876) and improved post-resection recurrence prediction [hazard ratio (HR) 3.179, 95% confidence interval (CI) 1.025-9.859] over that for the marker CA19-9 (HR 1.464; 95% CI 0.674-3.181). Capturing KRAS and GNAS may additionally present genetic proof in sufferers with IPMN-associated PDA and present process pancreatic surveillance.
Conclusions: Plasma cfDNA quantification by distinct measurements is helpful to foretell tumor burden. By acceptable strategies, dPCR-mediated mutation detection in sufferers with localized PDA and IPMN prone to progress to invasive carcinoma is possible and enhances typical biomarkers.
Description: ACE2 Antibody: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. Homology modeling shows 2019-nCoV has a similar receptor-binding domain structure as SARS-CoV, which suggests COVID-19 (2019-nCoV) may use ACE2 as a receptor in humans for infection. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.
Description: ACE2 Antibody: Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology. In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus. Homology modeling shows 2019-nCoV has a similar receptor-binding domain structure as SARS-CoV, which suggests COVID-19 (2019-nCoV) may use ACE2 as a receptor in humans for infection. The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function.
Description: A polyclonal antibody against PDE1B. Recognizes PDE1B from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against HSPA1B. Recognizes HSPA1B from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against GDV1. Recognizes GDV1 from Plasmodium relictum. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against sdeA. Recognizes sdeA from Legionella pneumophila subsp. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against MT2731. Recognizes MT2731 from Mycobacterium tuberculosis. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against ydhF. Recognizes ydhF from Escherichia coli. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against stxB. Recognizes stxB from Enterobacteria phage H19B. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against cagA. Recognizes cagA from Helicobacter pylori. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against IX. Recognizes IX from Enterobacteria phage M13. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against VII. Recognizes VII from Enterobacteria phage M13. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against gadA. Recognizes gadA from Escherichia coli. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against alaC. Recognizes alaC from Escherichia coli. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against glsA1. Recognizes glsA1 from Escherichia coli. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against III. Recognizes III from Enterobacteria phage M13. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against P. Recognizes P from Rabies virus. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against nrdB. Recognizes nrdB from Escherichia coli. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against pepP. Recognizes pepP from Mycoplasma pneumoniae. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against MPN_083. Recognizes MPN_083 from Mycoplasma pneumoniae. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against ldcA. Recognizes ldcA from Escherichia coli. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against ALTA1. Recognizes ALTA1 from Alternaria alternata. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against Perlwapin. Recognizes Perlwapin from Haliotis laevigata. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against GNAS. Recognizes GNAS from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against SKOR1. Recognizes SKOR1 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against Scytovirin. Recognizes Scytovirin from Scytonema varium. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against PRA1. Recognizes PRA1 from Candida albicans. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against GP. Recognizes GP from Zaire ebolavirus. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against rgpB. Recognizes rgpB from Porphyromonas gingivalis. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against GP. Recognizes GP from Zaire ebolavirus. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against lecA. Recognizes lecA from Pseudomonas aeruginosa. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against RNASEL. Recognizes RNASEL from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against p46. Recognizes p46 from Mycoplasma hyopneumoniae. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against GP. Recognizes GP from Zaire ebolavirus. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against dps. Recognizes dps from Mycobacterium smegmatis. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against BHRF1. Recognizes BHRF1 from Epstein-Barr virus. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against PIRT. Recognizes PIRT from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against p46. Recognizes p46 from Mycoplasma hyopneumoniae. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against p46. Recognizes p46 from Mycoplasma hyopneumoniae. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against ply. Recognizes ply from Streptococcus pneumoniae serotype 4. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against lktA. Recognizes lktA from Mannheimia haemolytica. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against pfo. Recognizes pfo from Clostridium perfringens. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against trxC. Recognizes trxC from Escherichia coli. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against speB. Recognizes speB from Streptococcus pyogenes. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against PMP2. Recognizes PMP2 from Horse. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against omcB. Recognizes omcB from Chlamydia trachomatis. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against eltB. Recognizes eltB from Escherichia coli. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against POLR2M. Recognizes POLR2M from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against PRSS56. Recognizes PRSS56 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
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