PCR-RFLP: a focused methodology to disclose host particular malacosporean an infection profiles (Cnidaria: Myxozoa: Malacosporea)
Malacosporeans are a bunch of endoparasitic cnidarians (Myxozoa) that use freshwater bryozoans and fish as last and intermediate hosts, respectively. The malacosporean Tetracapsuloides bryosalmonae causes proliferative kidney illness (PKD), an rising illness in aquaculture and wild fish populations, together with threatened salmonids in Europe and the USA.
Combined infections of malacosporeans are sometimes encountered, and a monitoring software for screening of a number of malacosporean species in both their fish or bryozoan hosts is due to this fact fascinating. We describe a cheap methodology that mixes PCR amplification of the partial 18S rRNA gene (~260 bp) and a single-step restriction fragment size polymorphism (RFLP) methodology for identification of 10 malacosporean lineages and species.
We display and take a look at this technique on a set of DNA extracted from malacosporeans infecting fish kidney and tissues sampled from bryozoan colonies and examine the outcomes with Sanger sequencing of the identical parasite DNA isolates. The PCR-RFLP and Sanger sequencing strategies agreed in 100% of circumstances. The PCR-RFLP methodology provides a variety of alternatives, together with screening giant panels of host tissue samples to achieve insights into an infection patterns, characterizing combined infections, and confirming extremely pathogenic T. bryosalmonae infections.
The strategy will also be additional refined as new sequence information turn into accessible for malacosporeans.
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, IHC;ELISA:1:1000-1:2000, IHC:1:10-1:50
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human, Mouse, Rat, Monkey. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/40000
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:10-1:50
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC, IF; Recommended dilution: WB:1:200-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Description: A polyclonal antibody for detection of TPH2 from Human, Mouse, Rat, Monkey. This TPH2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the non-phosphorylation site of S19
Description: A polyclonal antibody for detection of TPH2 from Human, Mouse, Rat, Monkey. This TPH2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the non-phosphorylation site of S19
Description: A polyclonal antibody for detection of TPH2 from Human, Mouse, Rat, Monkey. This TPH2 antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the non-phosphorylation site of S19
Description: This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.
Description: This gene encodes a member of the pterin-dependent aromatic acid hydroxylase family. The encoded protein catalyzes the first and rate limiting step in the biosynthesis of serotonin, an important hormone and neurotransmitter. Mutations in this gene may be associated with psychiatric diseases such as bipolar affective disorder and major depression.
Description: A polyclonal antibody against Phospho-TPH2 (Ser19). Recognizes Phospho-TPH2 (Ser19) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000
Description: A polyclonal antibody against TPH2 (Ab-19). Recognizes TPH2 (Ab-19) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: A polyclonal antibody against Phospho-TPH2 (S19). Recognizes Phospho-TPH2 (S19) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/40000
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against TPH2. Recognizes TPH2 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA
Description: A polyclonal antibody against Phospho-TPH2 (Ser19). Recognizes Phospho-TPH2 (Ser19) from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:1000
Description: A polyclonal antibody against TPH2 (Ab-19). Recognizes TPH2 (Ab-19) from Human, Mouse. This antibody is Unconjugated. Tested in the following application: ELISA, WB;WB:1:500-1:3000
Description: Human tryptophan_x000D_hydroxylase 2 (TPH2) also known as_x000D_neuronal tryptophan hydroxylase,_x000D_GenBank Accession No. NM_173353, a.a._x000D_151-466 (end) with an N-terminal His-tag,_x000D_MW = 37 kDa, expressed in an E. coli cell_x000D_expression system.
Description: A polyclonal antibody for detection of TPH2 phospho Ser19) from Human, Mouse, Rat. This TPH2 phospho Ser19) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the phosphorylation site of S19
Description: A polyclonal antibody for detection of TPH2 phospho Ser19) from Human, Mouse, Rat. This TPH2 phospho Ser19) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the phosphorylation site of S19
Description: A polyclonal antibody for detection of TPH2 phospho Ser19) from Human, Mouse, Rat. This TPH2 phospho Ser19) antibody is for WB, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from human TPH2 around the phosphorylation site of S19
Description: A Rabbit Polyclonal antibody against TPH2 (phospho Ser19) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: A Rabbit Polyclonal antibody against TPH2 (phospho Ser19) from Human/Mouse/Rat. This antibody is tested and validated for WB, ELISA, WB, ELISA
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human TPH2 (aa16-29) (N-Term). This antibody is tested and proven to work in the following applications:
Description: Description of target: isoform of tryptophan hydroxylase; may play a role in seratonin biosynthesis [RGD, Feb 2006];Species reactivity: Rat;Application: ELISA;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.56 ng/mL
Description: Description of target: Tryptophan hydroxylase (TPH; EC 1.14.16.4) is the rate-limiting enzyme in the synthesis of serotonin (5-hydroxytryptamine, or 5HT). 5HT is causally involved in numerous central nervous activities, and it has several functions in peripheral tissues, including the maintenance of vascular tone and gut motility.;Species reactivity: Human;Application: ELISA;Assay info: ;Sensitivity: < 0.119ng/mL
Description: The TPH2 Assay Kit is designed to measure TPH2 enzyme inhibition in a 96 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple - just mix two solutions, incubate, add quenching solution, and measure the fluorescence.
Description: The TPH2 Assay Kit is designed to measure TPH2 enzyme inhibition in a 384 reaction format. This fluorescence-based assay kit is especially suitable for high throughput screening applications. The procedure is quick, straightforward, and simple - just mix two solutions, incubate, add quenching solution, and measure the fluorescence.
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Tryptophan hydroxylase 2 / TPH2 (internal region). This antibody is tested and proven to work in the following applications:
Analysis of BOX-PCR and REP-PCR as Molecular Typing Instruments for Antarctic Streptomyces
Molecular research led to the resurgence of pure merchandise analysis from genus Streptomyces, already identified for his or her lengthy historical past and significance for the pharmaceutical trade. Nevertheless, species belonging to this genus are troublesome to establish and probably the most generally used strategies, that are primarily based on 16S rRNA sequencing, don’t discriminate between associated species. On this work, amplification profiles generated from BOX-PCR and REP-PCR of 49 Antarctic soil streptomycetes had been in comparison with consider the variety current within the group and to characterize the bacterial isolates, together with some 16S rRNA amplifications.
The BOX-A1R primer exhibit clearer amplification fragments, totally different from the amplification patterns obtained utilizing the REP 1R and 2R primers. A better variety was noticed with REP-PCR amplifications, regardless that a bigger variety of fragments was obtained with BOX-A1R primer amplifications. There are not less than 4 isolates that confirmed nice similarity (about 90%) in each strategies.
In different hand, there are two others which might be 90% related in BOX-PCR, however distant in REP-PCR, exhibiting solely 40% of similarity. Outcomes of the mixture of BOX-PCR and REP-PCR signify a easy and low-cost methodology to discriminate between Streptomyces strains. There isn’t any species identification with solely the 16S rRNA, most isolates appear to be associated to S. globisporus. Additional research added to the obtained outcomes could present higher information to assist the characterization of those microorganisms.
Digital PCR-based plasma cell-free DNA mutation evaluation for early-stage pancreatic tumor analysis and surveillance
Background: Cell-free DNA (cfDNA) shed from tumors into the circulation provides a software for most cancers detection. Right here, we evaluated the feasibility of cfDNA measurement and utility of digital PCR (dPCR)-based assays, which scale back subsampling error, for diagnosing pancreatic ductal adenocarcinoma (PDA) and surveillance of intraductal papillary mucinous neoplasm (IPMN).
Strategies: We collected plasma from seven establishments for cfDNA measurements. Scorching-spot mutations in KRAS and GNAS within the cfDNA from sufferers with PDA (n = 96), present process surveillance for IPMN (n = 112), and regular controls (n = 76) had been evaluated utilizing pre-amplification dPCR.
Outcomes: Upon Qubit measurement and duplicate quantity evaluation of hemoglobin-subunit (HBB) and mitochondrially encoded NADH:ubiquinone oxidoreductase core subunit 1 (MT-ND1) in plasma cfDNA, HBB provided the perfect decision between sufferers with PDA relative to wholesome topics [area under the curve (AUC) 0.862], whereas MT-ND1 revealed vital variations between IPMN and controls (AUC 0.851).
DPCR using pre-amplification cfDNA afforded correct tumor-derived mutant KRAS detection in plasma in resectable PDA (AUC 0.861-0.876) and improved post-resection recurrence prediction [hazard ratio (HR) 3.179, 95% confidence interval (CI) 1.025-9.859] over that for the marker CA19-9 (HR 1.464; 95% CI 0.674-3.181). Capturing KRAS and GNAS may additionally present genetic proof in sufferers with IPMN-associated PDA and present process pancreatic surveillance.
Conclusions: Plasma cfDNA quantification by distinct measurements is helpful to foretell tumor burden. By acceptable strategies, dPCR-mediated mutation detection in sufferers with localized PDA and IPMN prone to progress to invasive carcinoma is possible and enhances typical biomarkers.
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