May 24, 2022
lembcke.dk

Detection and characterization of Plasmodium spp.

Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR each in mosquito vectors and in people residing in traditionally endemic areas of Paraguay

lembcke.dk

Alpha-bungarotoxin, CF680r

9-00003
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF640r

9-00004
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF640r

9-00004
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF488a

9-00005
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF488a

9-00005
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF568

9-00006
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF568

9-00006
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF594

9-00007
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin CF594

9-00007
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF633

9-00009
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 500uG

Alpha-bungarotoxin, CF633

9-00009
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100uG
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin

00010-1 1MG
EUR 168
Description: Minimum order quantity: 1 unit of 1MG

Fluorescein-Alpha-bungarotoxin

00011 500uG
EUR 367
Description: Minimum order quantity: 1 unit of 500uG

Tetramethylrhodamine-Alpha-bungarotoxin

00012 500uG
EUR 383
Description: Minimum order quantity: 1 unit of 500uG

Fluorescein-alpha-bungarotoxin: (10x50ug)

00013 10ST
EUR 417
Description: Minimum order quantity: 1 unit of 10ST

Tetramethylrhodamine-alpha-bungarotoxin: (10x50ug)

00014 10ST
EUR 466
Description: Minimum order quantity: 1 unit of 10ST

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (500ug)

00015 500uG
EUR 466
Description: Minimum order quantity: 1 unit of 500uG

Sulforhodamine 101-a-bungarotoxin (Texas Red--a-bungarotoxin): (10x50ug)

00016 10ST
EUR 523
Description: Minimum order quantity: 1 unit of 10ST

Biotin-xx-a-bungarotoxin

00017 500uG
EUR 433
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 500uG

Alpha-Bungarotoxin, CF555 conjugate

9-00018
  • EUR 494.00
  • EUR 161.00
  • 500uG
  • 100ug
Description: Minimum order quantity: 1 unit of 100ug

Biotin-cAMP, diisopropylethylammonium salt:

00020 1MG
EUR 314
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cAMP, diisopropylethylammonium salt: (20x50ug)

00020-1 20ST
EUR 418
Description: Minimum order quantity: 1 unit of 20ST

Biotin-cGMP, diisopropylethylammonium salt:

00021 1MG
EUR 344
Description: Minimum order quantity: 1 unit of 1MG

Biotin-cGMP, diisopropylethylammonium salt: (20x50ug)

00021-1 20ST
EUR 448
Description: Minimum order quantity: 1 unit of 20ST

Cyanine 644-cAMP:

00022 1MG
EUR 491
Description: Minimum order quantity: 1 unit of 1MG

Cyanine 644-cAMP: (20x50ug)

00022-1 20ST
EUR 625
Description: Minimum order quantity: 1 unit of 20ST

Fluorescein Methotrexate, triammonium salt:

00023 1MG
EUR 281
Description: Minimum order quantity: 1 unit of 1MG

Staurosporine

00025 100uG
EUR 139
Description: Minimum order quantity: 1 unit of 100uG

Alpha-bungarotoxin, CF543

00026 500uG
EUR 494
Description: Minimum order quantity: 1 unit of 500uG

Rhodamine phalloidin 300u

00027 300
EUR 345
Description: Minimum order quantity: 1 unit of 300

Biotin-xx-phalloidin

00028 100U
EUR 428
Description: Minimum order quantity: 1 unit of 100U

Fluorescein-phalloidin

00030 300U
EUR 345
Description: Minimum order quantity: 1 unit of 300U

Rhodamine 110 phalloidin

00032 300ST
EUR 345
Description: Minimum order quantity: 1 unit of 300ST

Sulforhodamine 101 (Texas-Red) Phalloidin

00033 300EU
EUR 345
Description: Minimum order quantity: 1 unit of 300EU

Phalloidin, CF405M

00034 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF405M

00034-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

CF488A-cAMP

00036 100ug
EUR 172
Description: Minimum order quantity: 1 unit of 100ug

CF640R-cAMP

00037 100ug
EUR 172
Description: Minimum order quantity: 1 unit of 100ug

Phalloidin, CF555

00040 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF555

00040-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF647

00041 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Phalloidin, CF647

00041-T 50U
EUR 133
Description: Minimum order quantity: 1 unit of 50U

Phalloidin, CF488A

00042 300U
EUR 442
Description: Minimum order quantity: 1 unit of 300U

Suitability of pepper gentle mottle virus as a human enteric virus surrogate for assessing the efficacy of thermal or free-chlorine disinfection processes by utilizing infectivity assays and enhanced viability PCR

Evaluating the efficacy of disinfection processes to inactivate human enteric viruses is essential for the prevention and management of waterborne illnesses attributable to publicity to these viruses by way of ingesting water. Right here, we evaluated the inactivation of two consultant human enteric viruses (adenovirus kind 40 [AdV] and coxsackievirus B5 [CV]) by thermal or free-chlorine disinfection.
As well as, we in contrast the infectivity discount ratio of a plant virus (pepper gentle mottle virus [PMMoV], a lately proposed novel surrogate for human enteric viruses for the evaluation of virus elimination by coagulation‒fast sand filtration and membrane filtration) with that of the 2 human enteric viruses to evaluate the suitability of PMMoV as a human enteric virus surrogate to be used in thermal and free-chlorine disinfection processes.
Lastly, we examined whether or not standard or enhanced viability polymerase chain response (PCR) evaluation utilizing propidium monoazide (PMA) or improved PMA (PMAxx) with or with out an enhancer might be used as options to infectivity assays (i.e., plaque-forming unit methodology for AdV and CV; native lesion rely assay for PMMoV) for evaluating virus inactivation by disinfection processes. We discovered that PMMoV was extra proof against warmth therapy than AdV and CV, suggesting that PMMoV is a possible surrogate for these two enteric viruses with regard to thermal disinfection processes.
Nonetheless, PMMoV was rather more proof against chlorine therapy in contrast with AdV and CV (which is chlorine-resistant) (CT worth for 4-log10 inactivation: PMMoV, 84.5 mg-Cl2·min/L; CV, 1.15-1.19 mg-Cl2·min/L), suggesting that PMMoV shouldn’t be helpful as a surrogate for these enteric viruses with regard to free-chlorine disinfection processes.
For thermal disinfection, the magnitude of the sign discount noticed with PMAxx-Enhancer-PCR was comparable with the magnitude of discount in infectivity, indicating that PMAxx-Enhancer-PCR is a possible various to infectivity assay.
Nonetheless, for free-chlorine disinfection, the magnitude of the sign discount noticed with PMAxx-Enhancer-PCR was smaller than the magnitude of the discount in infectivity, indicating that PMAxx-Enhancer-PCR underestimated the efficacy of virus inactivation (i.e., overestimated the infectious virus focus) by chlorine therapy.
However, among the many PCR approaches examined within the current research (PCR alone, PMA-PCR or PMAxx-PCR both with or with out enhancer), PMAxx-Enhancer-PCR supplied probably the most correct evaluation of the efficacy of virus inactivation by thermal or free chlorine disinfection processes.

Detection of Leishmania infantum DNA by real-time PCR in saliva of canine

This research developed a real-time quantitative PCR (qPCR) assay to detect L. infantum kinetoplast DNA (kDNA) in canine saliva. The qPCR confirmed an effectivity of 93.8%, a coefficient of correlation of 0.996 and a detection restrict of 0.5 fg/response (0.005 parasites), though it detected till 0.25 fg/response (0.0025 parasites). When samples from 12 canine experimentally contaminated with L.
infantum have been collected, L. infantum kDNA was detected at 16-weeks post-infection (wpi) in 41.7% and 91.7% of saliva and bone marrow samples, respectively, and at 47-wpi in 75% of each samples. L. infantum kDNA might be detected by qPCR in canine saliva, with decrease sensitivity within the early phases of an infection and a decrease parasite load estimation in comparison with bone marrow.
Nonetheless, saliva had comparable sensitivities to bone marrow within the later phases of the an infection and might be used to detect L. infantum kDNA being conscious of its limitations.

cDNA from Human Tumor Tissue: Adrenal

C1235004-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Normal Adrenal Gland Whole tissue lysate

HALX-11050 1 mg
EUR 773

cDNA from Human Adult Normal Tissue: Adrenal

C1234004 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Adrenal

C1534004 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Adrenal

C1534004-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Bovine ADRENAL 50 EA*

57101-2 50 Each
EUR 756.65

Monkey (Cyno.) Normal Adrenal Gland Whole tissue lysate

MCL-1160 1 mg
EUR 773

Monkey (Rhesus) Normal Adrenal Gland Whole tissue lysate

MRL-1267 1 mg
EUR 773

cDNA from Human Diabetic Tissue: Whole Eye

C1236108Dia-10 10 reactions
EUR 383
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

BAM-12P, Bovine Adrenal Medulla Docosapeptide

5-00748 4 x 1mg Ask for price

cDNA from Human Adult Normal Tissue: Whole Eye

C1234108-10 10 reactions
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Whole Eye

C1534108-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Bovine EYE WHOLE 25 EA*

57111-2 25 EA
EUR 295.55

Bovine KIDNEY WHOLE 2 EA*

57120-2 2 EA
EUR 174.84

Bovine PITUITARY WHOLE 25 EA*

57133-2 25 EA
EUR 597.88

Paraffin Tissue Section - Human Adrenal Tumor: Adrenal Pheochromocytoma

T2235004-2 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Adrenal Dissociation System 9 (Adrenal), Mouse and Rat

4-20209 ea Ask for price

Adrenal Dissociation System 2 (Leydig, Adrenal), Mouse and Rat

4-20202 ea Ask for price

Pig ADRENAL 50 EA*

59401-2 50 EA
EUR 440.19

Rabbit ADRENAL 50 EA

41201-2 50 EA
EUR 341.23

Rabbit anti Whole Bovine serum antibody (IgG fraction)

40R-RB002 20 mg
EUR 327
Description: Affinity purified Rabbit polyclonal Rabbit anti Whole Bovine serum antibody (IgG fraction)

Adrenal Dissociation System 4 (Heart, Adrenal chromaffin, Paraneurons), Mouse and Rat

4-20204 ea Ask for price

Rabbit Anti-Bovine IgG (whole IgG from colostrum), unlabeled

80516 1 ml
EUR 202

Rabbit Anti-Bovine IgG (whole IgG from serum), unlabeled

80517 1 ml
EUR 202

Frozen Tissue Section - Lupus: Adrenal

T1236004Lup 5 slides
EUR 474
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Total Protein from Lupus: Adrenal

P1236004Lup 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Rabbit ADRENAL MATURE 50 EA

41301-2 50 EA
EUR 341.23

Histone (Whole) Antibody

abx023918-2ml 2 ml
EUR 509

Rat Whole Heart

PC35133 P0 Rat - Whole Heart X2
EUR 1341

Mouse Whole Heart

PC35135 P0 Mouse - Whole heart X2
EUR 1341

Evo? cDNA Kit

M1164-100
EUR 294

Evo? cDNA Kit

M1164-25
EUR 234

Novo? cDNA Kit

M1165-100
EUR 354

Novo? cDNA Kit

M1165-25
EUR 267

Evo? cDNA Supermix

M1168-100
EUR 381

Evo? cDNA Supermix

M1168-25
EUR 267

Novo? cDNA Supermix

M1169-100
EUR 441

Novo? cDNA Supermix

M1169-25
EUR 289

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Human adrenal cortex antibody ELISA Kit

ELA-E0342h 96 Tests
EUR 824

Frozen Tissue Section - Human Tumor: Adrenal

T1235004 5 slides
EUR 338
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adrenal Tumor

T2235004 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Total Protein from Liver Cirrhosis: Adrenal

P1236004Lcs 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 871.00
  • EUR 662.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

Human adrenal cortex antibody,ACA ELISA Kit

CN-03832H1 96T
EUR 452

Human adrenal cortex antibody,ACA ELISA Kit

CN-03832H2 48T
EUR 302

Human adrenal cortex antibody,ACA ELISA kit

E01A2028-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human adrenal cortex antibody,ACA ELISA kit

E01A2028-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human adrenal cortex antibody,ACA ELISA kit

E01A2028-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat adrenal cortex antibody,ACA ELISA kit

E02A2028-192T 192 tests
EUR 1270
Description: A competitive ELISA for quantitative measurement of Rat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat adrenal cortex antibody,ACA ELISA kit

E02A2028-48 1 plate of 48 wells
EUR 520
Description: A competitive ELISA for quantitative measurement of Rat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat adrenal cortex antibody,ACA ELISA kit

E02A2028-96 1 plate of 96 wells
EUR 685
Description: A competitive ELISA for quantitative measurement of Rat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Frozen Tissue Section - Human Adult Normal: Adrenal

T1234004 5 slides
EUR 349
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section - Human Diabetic Tissue: Adrenal

T1236004Dia 5 slides
EUR 465
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adult Normal: Adrenal

T2234004 5 slides
EUR 211
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adrenal Tumor: Adenoma

T2235004-1 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Leave a Reply

Your email address will not be published.