by Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR each in mosquito vectors and in people residing in traditionally endemic areas of Paraguay
In Paraguay, no instances of Malaria have been recorded since 2011. Microscopy and the SnM-PCR approach have been applied to detect and characterize Plasmodium spp. each in mosquitoes and in people residing in traditionally endemic areas of Paraguay, to guage the potential of discovering asymptomatic instances and/or Plasmodium parasites circulating in anophelines.
Between 2013 and 2015, 361 human blood samples have been collected on filter paper, and between 2016 and 2017, 938 feminine Anopheles mosquitoes have been captured in 15 Paraguayan localities. All of the diagnostic methods confirmed adverse outcomes. We noticed no asymptomatic case or Plasmodium circulating in vectors.
Product not found
Suitability of pepper gentle mottle virus as a human enteric virus surrogate for assessing the efficacy of thermal or free-chlorine disinfection processes by utilizing infectivity assays and enhanced viability PCR
Evaluating the efficacy of disinfection processes to inactivate human enteric viruses is essential for the prevention and management of waterborne illnesses attributable to publicity to these viruses by way of ingesting water. Right here, we evaluated the inactivation of two consultant human enteric viruses (adenovirus kind 40 [AdV] and coxsackievirus B5 [CV]) by thermal or free-chlorine disinfection.
As well as, we in contrast the infectivity discount ratio of a plant virus (pepper gentle mottle virus [PMMoV], a lately proposed novel surrogate for human enteric viruses for the evaluation of virus elimination by coagulation‒fast sand filtration and membrane filtration) with that of the 2 human enteric viruses to evaluate the suitability of PMMoV as a human enteric virus surrogate to be used in thermal and free-chlorine disinfection processes.
Lastly, we examined whether or not standard or enhanced viability polymerase chain response (PCR) evaluation utilizing propidium monoazide (PMA) or improved PMA (PMAxx) with or with out an enhancer might be used as options to infectivity assays (i.e., plaque-forming unit methodology for AdV and CV; native lesion rely assay for PMMoV) for evaluating virus inactivation by disinfection processes. We discovered that PMMoV was extra proof against warmth therapy than AdV and CV, suggesting that PMMoV is a possible surrogate for these two enteric viruses with regard to thermal disinfection processes.
Nonetheless, PMMoV was rather more proof against chlorine therapy in contrast with AdV and CV (which is chlorine-resistant) (CT worth for 4-log10 inactivation: PMMoV, 84.5 mg-Cl2·min/L; CV, 1.15-1.19 mg-Cl2·min/L), suggesting that PMMoV shouldn’t be helpful as a surrogate for these enteric viruses with regard to free-chlorine disinfection processes.
For thermal disinfection, the magnitude of the sign discount noticed with PMAxx-Enhancer-PCR was comparable with the magnitude of discount in infectivity, indicating that PMAxx-Enhancer-PCR is a possible various to infectivity assay.
Nonetheless, for free-chlorine disinfection, the magnitude of the sign discount noticed with PMAxx-Enhancer-PCR was smaller than the magnitude of the discount in infectivity, indicating that PMAxx-Enhancer-PCR underestimated the efficacy of virus inactivation (i.e., overestimated the infectious virus focus) by chlorine therapy.
However, among the many PCR approaches examined within the current research (PCR alone, PMA-PCR or PMAxx-PCR both with or with out enhancer), PMAxx-Enhancer-PCR supplied probably the most correct evaluation of the efficacy of virus inactivation by thermal or free chlorine disinfection processes.
Detection of Leishmania infantum DNA by real-time PCR in saliva of canine
This research developed a real-time quantitative PCR (qPCR) assay to detect L. infantum kinetoplast DNA (kDNA) in canine saliva. The qPCR confirmed an effectivity of 93.8%, a coefficient of correlation of 0.996 and a detection restrict of 0.5 fg/response (0.005 parasites), though it detected till 0.25 fg/response (0.0025 parasites). When samples from 12 canine experimentally contaminated with L.
infantum have been collected, L. infantum kDNA was detected at 16-weeks post-infection (wpi) in 41.7% and 91.7% of saliva and bone marrow samples, respectively, and at 47-wpi in 75% of each samples. L. infantum kDNA might be detected by qPCR in canine saliva, with decrease sensitivity within the early phases of an infection and a decrease parasite load estimation in comparison with bone marrow.
Nonetheless, saliva had comparable sensitivities to bone marrow within the later phases of the an infection and might be used to detect L. infantum kDNA being conscious of its limitations.
 Rat Adrenal Whole tissue lysate |
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| RAL-1470 | Alpha Diagnostics | 100ug | EUR 213 |
 cDNA from Human Tumor Tissue: Adrenal |
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| C1235004-10 | Biochain | 10 reactions | EUR 282 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Human Normal Adrenal Gland Whole tissue lysate |
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| HALX-11050 | Alpha Diagnostics | 1 mg | EUR 773 |
 cDNA from Human Adult Normal Tissue: Adrenal |
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| C1234004 | Biochain | 40 reactions | EUR 376 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Normal Tissue: Adrenal) cDNA from Monkey (Rhesus) Normal Tissue: Adrenal |
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| C1534004 | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Normal Tissue: Adrenal) cDNA from Monkey (Cynomolgus) Normal Tissue: Adrenal |
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| C1534004-Cy | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Normal Adrenal Gland Whole tissue lysate) Monkey (Rhesus) Normal Adrenal Gland Whole tissue lysate |
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| MRL-1267 | Alpha Diagnostics | 1 mg | EUR 773 |
 Normal Adrenal Gland Whole tissue lysate) Monkey (Cyno.) Normal Adrenal Gland Whole tissue lysate |
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| MCL-1160 | Alpha Diagnostics | 1 mg | EUR 773 |
 cDNA from Human Diabetic Tissue: Whole Eye |
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| C1236108Dia-10 | Biochain | 10 reactions | EUR 383 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 BAM-12P, Bovine Adrenal Medulla Docosapeptide |
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| 5-00748 | CHI Scientific | 4 x 1mg | Ask for price |
 cDNA from Human Adult Normal Tissue: Whole Eye |
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| C1234108-10 | Biochain | 10 reactions | EUR 446 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Normal Tissue: Whole Eye) cDNA from Monkey (Cynomolgus) Normal Tissue: Whole Eye |
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| C1534108-Cy | Biochain | 40 reactions | EUR 540 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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, Mouse and Rat) Adrenal Dissociation System 9 (Adrenal), Mouse and Rat |
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| 4-20209 | CHI Scientific | ea | Ask for price |
 Paraffin Tissue Section - Human Adrenal Tumor: Adrenal Pheochromocytoma |
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| T2235004-2 | Biochain | 5 slides | EUR 257 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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) Rabbit anti Whole Bovine serum antibody (IgG fraction) |
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| 40R-RB002 | Fitzgerald | 20 mg | EUR 327 |
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Description: Affinity purified Rabbit polyclonal Rabbit anti Whole Bovine serum antibody (IgG fraction) |
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, Mouse and Rat) Adrenal Dissociation System 2 (Leydig, Adrenal), Mouse and Rat |
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| 4-20202 | CHI Scientific | ea | Ask for price |
, unlabeled) Rabbit Anti-Bovine IgG (whole IgG from colostrum), unlabeled |
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| 80516 | Alpha Diagnostics | 1 ml | EUR 202 |
, unlabeled) Rabbit Anti-Bovine IgG (whole IgG from serum), unlabeled |
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| 80517 | Alpha Diagnostics | 1 ml | EUR 202 |
, Mouse and Rat) Adrenal Dissociation System 4 (Heart, Adrenal chromaffin, Paraneurons), Mouse and Rat |
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| 4-20204 | CHI Scientific | ea | Ask for price |
 Antibody) Histone (Whole) Antibody |
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| abx023918-2ml | Abbexa | 2 ml | EUR 509 |
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 Rat Whole Heart |
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| PC35133 | Neuromics | P0 Rat - Whole Heart X2 | EUR 1341 |
 Mouse Whole Heart |
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| PC35135 | Neuromics | P0 Mouse - Whole heart X2 | EUR 1341 |
 Total Protein from Lupus: Adrenal |
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| P1236004Lup | Biochain | 1 mg | EUR 461 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 Frozen Tissue Section - Lupus: Adrenal |
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| T1236004Lup | Biochain | 5 slides | EUR 474 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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 cDNA Synthesis SuperMix |
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| 20-abx09801420ulSystems | Abbexa |
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 Evo? cDNA Kit |
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| M1164-100 | Biovision | EUR 294 | |
Evo? cDNA Kit |
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| M1164-25 | Biovision | EUR 234 | |
 Novo? cDNA Kit |
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| M1165-100 | Biovision | EUR 354 | |
Novo? cDNA Kit |
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| M1165-25 | Biovision | EUR 267 | |
 Evo? cDNA Supermix |
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| M1168-100 | Biovision | EUR 381 | |
Evo? cDNA Supermix |
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| M1168-25 | Biovision | EUR 267 | |
 Novo? cDNA Supermix |
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| M1169-100 | Biovision | EUR 441 | |
Novo? cDNA Supermix |
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| M1169-25 | Biovision | EUR 289 | |
 Human adrenal cortex antibody ELISA Kit |
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| ELA-E0342h | Lifescience Market | 96 Tests | EUR 824 |
 Total Protein from Liver Cirrhosis: Adrenal |
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| P1236004Lcs | Biochain | 1 mg | EUR 461 |
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Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation. |
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 Frozen Tissue Section - Human Tumor: Adrenal |
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| T1235004 | Biochain | 5 slides | EUR 338 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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 Paraffin Tissue Section - Human Adrenal Tumor |
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| T2235004 | Biochain | 5 slides | EUR 257 |
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Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
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) CMV protein (whole cell) |
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| 30-AC70 | Fitzgerald | 1 ml | EUR 403 |
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Description: Purified native CMV protein (whole cell) |
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) Monkey IgM (whole molecule) |
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| 31R-AI093 | Fitzgerald | 250 µg | EUR 417 |
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Description: Monkey whole IgM |
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 Whole Human Serum antibody |
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| 20-1727 | Fitzgerald | 1 L | EUR 30 |
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Description: Goat polyclonal Whole Human Serum antibody |
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Whole Human Serum antibody |
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| 20-S1110G000-V0 | Fitzgerald | 10 ml | EUR 133 |
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Description: Goat polyclonal Whole Human Serum antibody |
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 Whole Blood PCR Mix |
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| M1143-200 | Biovision | EUR 321 | |
 cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis |
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| C1634310 | Biochain | 40 reactions | EUR 621 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 cDNA from Plant Normal Tissue: cDNA from Plant: Corn |
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| C1634330 | Biochain | 40 reactions | EUR 621 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 cDNA from Plant Normal Tissue: cDNA from Plant: Orange |
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| C1634340 | Biochain | 40 reactions | EUR 621 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 cDNA from Plant Normal Tissue: cDNA from Plant: Potato |
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| C1634350 | Biochain | 40 reactions | EUR 621 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 cDNA from Plant Normal Tissue: cDNA from Plant: Rice |
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| C1634360 | Biochain | 40 reactions | EUR 621 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 cDNA from Plant Normal Tissue: cDNA from Plant: Wheat |
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| C1634390 | Biochain | 40 reactions | EUR 621 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 IgG aff pure) Goat Anti-human Chorionic Gonadotropin Whole (hCG-Whole) IgG aff pure |
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| HCG14-A | Alpha Diagnostics | 1 mg | EUR 286 |
) First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb) |
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| 20-abx09801620ulSystems | Abbexa |
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) First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb) |
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| 20-abx09802120ulSystems | Abbexa |
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 cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean |
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| C1634370 | Biochain | 40 reactions | EUR 621 |
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Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
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 Human adrenal cortex antibody,ACA ELISA Kit |
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| 201-12-0523 | SunredBio | 96 tests | EUR 440 |
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Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
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 Human adrenal cortex antibody, ACA ELISA Kit |
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| CSB-E04977h-24T | Cusabio | 1 plate of 24 wells | EUR 165 |
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Description: Quantitative sandwich ELISA kit for measuring Human adrenal cortex antibody, ACA in samples from serum, plasma, cell culture supernates, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price. |
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Human adrenal cortex antibody, ACA ELISA Kit |
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| 1-CSB-E04977h | Cusabio |
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Description: Quantitative sandwich ELISA kit for measuring Human adrenal cortex antibody, ACA in samples from serum, plasma, cell culture supernates, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits. |
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 Human adrenal cortex antibody,ACA ELISA kit |
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| E01A2028-192T | B-Gene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human adrenal cortex antibody,ACA ELISA kit |
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| E01A2028-48 | B-Gene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Human adrenal cortex antibody,ACA ELISA kit |
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| E01A2028-96 | B-Gene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Mouse adrenal cortex antibody,ACA ELISA kit |
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| E03A2028-192T | B-Gene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Mouse adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse adrenal cortex antibody,ACA ELISA kit |
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| E03A2028-48 | B-Gene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Mouse adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Mouse adrenal cortex antibody,ACA ELISA kit |
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| E03A2028-96 | B-Gene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Mouse adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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 Goat adrenal cortex antibody,ACA ELISA kit |
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| E06A2028-192T | B-Gene | 192 tests | EUR 1270 |
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Description: A competitive ELISA for quantitative measurement of Goat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat adrenal cortex antibody,ACA ELISA kit |
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| E06A2028-48 | B-Gene | 1 plate of 48 wells | EUR 520 |
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Description: A competitive ELISA for quantitative measurement of Goat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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Goat adrenal cortex antibody,ACA ELISA kit |
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| E06A2028-96 | B-Gene | 1 plate of 96 wells | EUR 685 |
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Description: A competitive ELISA for quantitative measurement of Goat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species. |
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