March 5, 2021
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Enzymes / Equipments / Medium & Serums / Pcr Kits / protective / Reagents / Recombinant Proteins

Detection and characterization of Plasmodium spp.

Bjornby Bjorn

Detection and characterization of Plasmodium spp. by semi-nested multiplex PCR each in mosquito vectors and in people residing in traditionally endemic areas of Paraguay

In Paraguay, no instances of Malaria have been recorded since 2011. Microscopy and the SnM-PCR approach have been applied to detect and characterize Plasmodium spp. each in mosquitoes and in people residing in traditionally endemic areas of Paraguay, to guage the potential of discovering asymptomatic instances and/or Plasmodium parasites circulating in anophelines.
Between 2013 and 2015, 361 human blood samples have been collected on filter paper, and between 2016 and 2017, 938 feminine Anopheles mosquitoes have been captured in 15 Paraguayan localities. All of the diagnostic methods confirmed adverse outcomes. We noticed no asymptomatic case or Plasmodium circulating in vectors.

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Suitability of pepper gentle mottle virus as a human enteric virus surrogate for assessing the efficacy of thermal or free-chlorine disinfection processes by utilizing infectivity assays and enhanced viability PCR

Evaluating the efficacy of disinfection processes to inactivate human enteric viruses is essential for the prevention and management of waterborne illnesses attributable to publicity to these viruses by way of ingesting water. Right here, we evaluated the inactivation of two consultant human enteric viruses (adenovirus kind 40 [AdV] and coxsackievirus B5 [CV]) by thermal or free-chlorine disinfection.
As well as, we in contrast the infectivity discount ratio of a plant virus (pepper gentle mottle virus [PMMoV], a lately proposed novel surrogate for human enteric viruses for the evaluation of virus elimination by coagulation‒fast sand filtration and membrane filtration) with that of the 2 human enteric viruses to evaluate the suitability of PMMoV as a human enteric virus surrogate to be used in thermal and free-chlorine disinfection processes.
Lastly, we examined whether or not standard or enhanced viability polymerase chain response (PCR) evaluation utilizing propidium monoazide (PMA) or improved PMA (PMAxx) with or with out an enhancer might be used as options to infectivity assays (i.e., plaque-forming unit methodology for AdV and CV; native lesion rely assay for PMMoV) for evaluating virus inactivation by disinfection processes. We discovered that PMMoV was extra proof against warmth therapy than AdV and CV, suggesting that PMMoV is a possible surrogate for these two enteric viruses with regard to thermal disinfection processes.
Nonetheless, PMMoV was rather more proof against chlorine therapy in contrast with AdV and CV (which is chlorine-resistant) (CT worth for 4-log10 inactivation: PMMoV, 84.5 mg-Cl2·min/L; CV, 1.15-1.19 mg-Cl2·min/L), suggesting that PMMoV shouldn’t be helpful as a surrogate for these enteric viruses with regard to free-chlorine disinfection processes.
For thermal disinfection, the magnitude of the sign discount noticed with PMAxx-Enhancer-PCR was comparable with the magnitude of discount in infectivity, indicating that PMAxx-Enhancer-PCR is a possible various to infectivity assay.
Nonetheless, for free-chlorine disinfection, the magnitude of the sign discount noticed with PMAxx-Enhancer-PCR was smaller than the magnitude of the discount in infectivity, indicating that PMAxx-Enhancer-PCR underestimated the efficacy of virus inactivation (i.e., overestimated the infectious virus focus) by chlorine therapy.
However, among the many PCR approaches examined within the current research (PCR alone, PMA-PCR or PMAxx-PCR both with or with out enhancer), PMAxx-Enhancer-PCR supplied probably the most correct evaluation of the efficacy of virus inactivation by thermal or free chlorine disinfection processes.

Detection of Leishmania infantum DNA by real-time PCR in saliva of canine

This research developed a real-time quantitative PCR (qPCR) assay to detect L. infantum kinetoplast DNA (kDNA) in canine saliva. The qPCR confirmed an effectivity of 93.8%, a coefficient of correlation of 0.996 and a detection restrict of 0.5 fg/response (0.005 parasites), though it detected till 0.25 fg/response (0.0025 parasites). When samples from 12 canine experimentally contaminated with L.
infantum have been collected, L. infantum kDNA was detected at 16-weeks post-infection (wpi) in 41.7% and 91.7% of saliva and bone marrow samples, respectively, and at 47-wpi in 75% of each samples. L. infantum kDNA might be detected by qPCR in canine saliva, with decrease sensitivity within the early phases of an infection and a decrease parasite load estimation in comparison with bone marrow.
Nonetheless, saliva had comparable sensitivities to bone marrow within the later phases of the an infection and might be used to detect L. infantum kDNA being conscious of its limitations.

Rat Adrenal Whole tissue lysate
RAL-1470 100ug
EUR 213

cDNA from Human Tumor Tissue: Adrenal
C1235004-10 10 reactions
EUR 282
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Normal Adrenal Gland Whole tissue lysate
HALX-11050 1 mg
EUR 773

cDNA from Human Adult Normal Tissue: Adrenal
C1234004 40 reactions
EUR 376
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Adrenal
C1534004 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Adrenal
C1534004-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Monkey (Rhesus) Normal Adrenal Gland Whole tissue lysate
MRL-1267 1 mg
EUR 773

Monkey (Cyno.) Normal Adrenal Gland Whole tissue lysate
MCL-1160 1 mg
EUR 773

cDNA from Human Diabetic Tissue: Whole Eye
C1236108Dia-10 10 reactions
EUR 383
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Human Adult Normal Tissue: Whole Eye
C1234108-10 10 reactions
EUR 446
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Cynomolgus) Normal Tissue: Whole Eye
C1534108-Cy 40 reactions
EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

BAM-12P, Bovine Adrenal Medulla Docosapeptide
5-00748 4 x 1mg Ask for price

Adrenal Dissociation System 9 (Adrenal), Mouse and Rat
4-20209 ea Ask for price

Paraffin Tissue Section - Human Adrenal Tumor: Adrenal Pheochromocytoma
T2235004-2 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Rabbit anti Whole Bovine serum antibody (IgG fraction)
40R-RB002 20 mg
EUR 327
Description: Affinity purified Rabbit polyclonal Rabbit anti Whole Bovine serum antibody (IgG fraction)

Adrenal Dissociation System 2 (Leydig, Adrenal), Mouse and Rat
4-20202 ea Ask for price

Rabbit Anti-Bovine IgG (whole IgG from colostrum), unlabeled
80516 1 ml
EUR 202

Rabbit Anti-Bovine IgG (whole IgG from serum), unlabeled
80517 1 ml
EUR 202

Adrenal Dissociation System 4 (Heart, Adrenal chromaffin, Paraneurons), Mouse and Rat
4-20204 ea Ask for price

Histone (Whole) Antibody
abx023918-2ml 2 ml
EUR 509
  • Shipped within 5-10 working days.

Rat Whole Heart
PC35133 P0 Rat - Whole Heart X2
EUR 1341

Mouse Whole Heart
PC35135 P0 Mouse - Whole heart X2
EUR 1341

Total Protein from Lupus: Adrenal
P1236004Lup 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Frozen Tissue Section - Lupus: Adrenal
T1236004Lup 5 slides
EUR 474
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

cDNA Synthesis SuperMix
20-abx09801420ulSystems
  • EUR 565.00
  • EUR 481.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Evo? cDNA Kit
M1164-100
EUR 294

Evo? cDNA Kit
M1164-25
EUR 234

Novo? cDNA Kit
M1165-100
EUR 354

Novo? cDNA Kit
M1165-25
EUR 267

Evo? cDNA Supermix
M1168-100
EUR 381

Evo? cDNA Supermix
M1168-25
EUR 267

Novo? cDNA Supermix
M1169-100
EUR 441

Novo? cDNA Supermix
M1169-25
EUR 289

Human adrenal cortex antibody ELISA Kit
ELA-E0342h 96 Tests
EUR 824

Total Protein from Liver Cirrhosis: Adrenal
P1236004Lcs 1 mg
EUR 461
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Frozen Tissue Section - Human Tumor: Adrenal
T1235004 5 slides
EUR 338
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Adrenal Tumor
T2235004 5 slides
EUR 257
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

CMV protein (whole cell)
30-AC70 1 ml
EUR 403
Description: Purified native CMV protein (whole cell)

Monkey IgM (whole molecule)
31R-AI093 250 µg
EUR 417
Description: Monkey whole IgM

Whole Human Serum antibody
20-1727 1 L
EUR 30
Description: Goat polyclonal Whole Human Serum antibody

Whole Human Serum antibody
20-S1110G000-V0 10 ml
EUR 133
Description: Goat polyclonal Whole Human Serum antibody

Whole Blood PCR Mix
M1143-200
EUR 321

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis
C1634310 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn
C1634330 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange
C1634340 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato
C1634350 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice
C1634360 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat
C1634390 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Goat Anti-human Chorionic Gonadotropin Whole (hCG-Whole) IgG aff pure
HCG14-A 1 mg
EUR 286

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean
C1634370 40 reactions
EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
20-abx09801620ulSystems
  • EUR 620.00
  • EUR 523.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)
20-abx09802120ulSystems
  • EUR 871.00
  • EUR 662.00
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Goat adrenal cortex antibody,ACA ELISA kit
E06A2028-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat adrenal cortex antibody,ACA ELISA kit
E06A2028-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat adrenal cortex antibody,ACA ELISA kit
E06A2028-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human adrenal cortex antibody,ACA ELISA kit
E01A2028-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human adrenal cortex antibody,ACA ELISA kit
E01A2028-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human adrenal cortex antibody,ACA ELISA kit
E01A2028-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse adrenal cortex antibody,ACA ELISA kit
E03A2028-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse adrenal cortex antibody,ACA ELISA kit
E03A2028-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse adrenal cortex antibody,ACA ELISA kit
E03A2028-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit adrenal cortex antibody,ACA ELISA kit
E04A2028-192T 192 tests
EUR 1270
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit adrenal cortex antibody,ACA ELISA kit
E04A2028-48 1 plate of 48 wells
EUR 520
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit adrenal cortex antibody,ACA ELISA kit
E04A2028-96 1 plate of 96 wells
EUR 685
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit adrenal cortex antibody,ACA in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Bjorn

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